Genetic diversity and structure of Brazilian ginger germplasm (Zingiber officinale) revealed by AFLP markers
Genetic diversity and structure of Brazilian ginger germplasm (Zingiber officinale) revealed by AFLP markers
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DOI：
10.1007/s10709-016-9930-1
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发表时间：
2016-10
期刊：
影响因子：
1.5
通讯作者：
Eleonora Zambrano Blanco;M. Bajay;M. Siqueira;M. Zucchi;J. B. Pinheiro
中科院分区：
文献类型：
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作者：
Eleonora Zambrano Blanco;M. Bajay;M. Siqueira;M. Zucchi;J. B. Pinheiro
生姜是一种具有药用和烹饪特性的蔬菜，广泛种植于巴西南部和东南部。姜属植物遗传变异的研究对于指导今后的保护和遗传改良研究具有重要意义，但在巴西，姜属植物遗传变异的研究还很少。利用AFLP分子标记技术对55份巴西姜和6份哥伦比亚姜的遗传多样性和遗传结构进行了分析。分子特征分析基于13个引物组合，平均产生113.5个多态性位点。Nei（Hj），Shannon-Weiner指数（I）和有效等位基因数（ne）的遗传多样性估计在哥伦比亚加入巴西加入。分子方差分析表明，大多数遗传变异发生在两国之间，而在巴西人群中没有遗传结构，可能每个地区都有100%的遗传变异。的baidu模型为基础的聚类和树状图使用的相异的系数Jaccard是一致的彼此，并表明，巴西加入是高度相似的本身之间，无论地理区域的起源。我们认为，种间变异的探索和引进新品种的Z. officinale是在巴西育种计划中产生多样性的可行替代方案。引进新的遗传材料肯定有助于提高这种作物的遗传基础。
Ginger is a vegetable with medicinal and culinary properties widely cultivated in the Southern and Southeastern Brazil. The knowledge of ginger species’ genetic variability is essential to direct correctly future studies of conservation and genetic improvement, but in Brazil, little is known about this species’ genetic variability. In this study, we analyzed the genetic diversity and structure of 55 Brazilian accessions and 6 Colombian accessions of ginger, using AFLP (Amplified Fragment Length Polymorphism) molecular markers. The molecular characterization was based on 13 primers combinations, which generated an average of 113.5 polymorphic loci. The genetic diversity estimates of Nei (Hj), Shannon–Weiner index (I) and an effective number of alleles (ne) were greater in the Colombian accessions in relation to the Brazilian accessions. The analysis of molecular variance showed that most of the genetic variation occurred between the two countries while in the Brazilian populations there is no genetic structure and probably each region harbors 100 % of genetic variation found in the samples. The bayesian model-based clustering and the dendrogram using the dissimilarity’s coefficient of Jaccard were congruent with each other and showed that the Brazilian accessions are highly similar between themselves, regardless of the geographic region of origin. We suggested that the exploration of the interspecific variability and the introduction of new varieties ofZ.officinaleare viable alternatives for generating diversity in breeding programs in Brazil. The introduction of new genetic materials will certainly contribute to a higher genetic basis of such crop.