Glomerular localization of erythropoietin receptor mRNA and protein in neonatal and mature mouse kidney
Glomerular localization of erythropoietin receptor mRNA and protein in neonatal and mature mouse kidney
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DOI：
10.1159/000084109
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发表时间：
2005-01-01
影响因子：
--
通讯作者：
Sasaki, S
中科院分区：
文献类型：
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作者：
Echigoya, MH;Obikane, K;Sasaki, S
背景/目标：促红细胞生成素（EPO）具有公认的造血特性，是通过与其受体（EPO-R）结合而形成红细胞的主要刺激物。最近的证据表明EPO在包括肾脏在内的几种非造血组织中的病理生理作用。我们的目的是进一步阐明EPO-R在正常肾脏中的肾小球定位，以及其在肾小球形成过程中的表达变化。方法：采用原位杂交和免疫组织化学方法，检测新生和成年小鼠肾脏EPO-R mRNA和蛋白的表达。为了证实在成熟和发育的肾小球足细胞中EPO-R表达的精确定位和发育变化，我们检查了足细胞标记物WT-1和突触足蛋白的共表达。结果如下：除了最近报道的肾小管表达外，还在成年小鼠肾小球足细胞和毛细血管内细胞中观察到EPO-R表达。在新生肾中，EPO-R mRNA和蛋白表达首先在发育中的足细胞中观察到S形小体，随后在毛细血管环和成熟阶段的肾小球中表达增加。免疫电镜观察显示，在毛细血管袢晚期和成熟期，肾小球足细胞基底侧有EPO-R的胞质表达。结论：EPO-R在小鼠肾脏发育和成熟的足细胞中均有表达，提示EPO可能在足细胞生物学中发挥作用。版权所有（C）2005 S. Karger AG，巴塞尔。
Background/Aims: Erythropoietin (EPO) possesses well-established hematopoietic properties as the primary stimulator of red blood cell formation by binding to its receptor (EPO-R). Recent evidence suggests pathophysiological roles of EPO in several non-hematopoietic tissues including kidney. Our aim was to further clarify the glomerular localization of EPO-R in normal kidney, as well as changes in its expression during glomerulogenesis. Methods: We analyzed EPO-R mRNA and protein expression in neonatal and adult mouse kidney by in situ hybridization and immunohistochemistry. To confirm the precise localization and developmental changes of EPO-R expression in podocytes in mature and developing glomeruli, we examined co-expression with the podocyte markers WT-1 and synaptopodin. Results: In addition to tubular expression as reported recently, EPO-R expression was observed in podocytes as well as endocapillary cells in the glomeruli from adult mice. In newborn kidney, EPO-R mRNA and protein expression was first observed in developing podocytes in S-shaped bodies with expression subsequently increasing in glomeruli at the capillary-loop and maturing stages. Immunoelectron microscopy also demonstrated cytoplasmic expression of EPO-R that was prominent at the basal sides of podocytes in glomeruli at the late capillary-loop and maturing stage. Conclusion: EPO-R is expressed in developing and mature podocytes in mouse kidney, suggesting a possible role for EPO in podocyte biology. Copyright (C) 2005 S. Karger AG, Basel.