The yes proto-oncogene is present in amphibians and contributes to the maternal RNA pool in the oocyte.
The yes proto-oncogene is present in amphibians and contributes to the maternal RNA pool in the oocyte.
复制标题
原癌基因存在于两栖动物中，并为卵母细胞中的母体 RNA 库做出贡献。
DOI：
--
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Fero
中科院分区：
文献类型：
--
作者：
R. Steele;M. Irwin;C. Knudsen;J. W. Collett;J. Fero
利用病毒癌基因探针对非洲爪蟾（Xenopus laevis）的cDNA文库进行低严格筛选，分离出具有限制性内切图的克隆，这些克隆与先前鉴定的非洲爪蟾src cDNA克隆不同。由其中一个克隆合成的RNA体外翻译产生的多肽的大小略大于X. laevis pp60src，但其蛋白酶裂解模式与pp60src产生的几乎相同。对该克隆的DNA序列测定表明，该克隆来源于一种野田鼠基因同源物。在子房中发现了yes基因的转录本，包括一个主要的3.4 kb种和一些次要的种。这些发现表明，分离src和yes基因的重复事件发生在两栖动物出现之前，yes基因产物可能在卵子发生或早期发育中起重要作用。
Low stringency screening of a cDNA library from the frog Xenopus laevis with a viral yes oncogene probe resulted in the isolation of clones with restriction maps which were distinct from previously identified X. laevis src cDNA clones. The polypeptide produced by in vitro translation of RNA synthesized from one of the clones was slightly larger in size than X. laevis pp60src, but gave protease cleavage patterns which were nearly identical to those yielded by pp60src. Determination of the DNA sequence of this clone revealed that it is derived from a X. laevis yes gene homologue. Transcripts from the yes gene were found in the ovary and consisted of a major 3.4 kb species and additional minor species. These findings indicate that the duplication event separating the src and yes genes occurred prior to the appearance of amphibians and that the yes gene product is likely to play an important role in oogenesis or early development.