Identification and characterization of microRNAs expressed in human breast cancer T-47D cells in response to prolactin treatment by Solexa deep-sequencing technology.
Identification and characterization of microRNAs expressed in human breast cancer T-47D cells in response to prolactin treatment by Solexa deep-sequencing technology.
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DOI：
10.1016/j.bbrc.2013.02.016
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发表时间：
2013-03
影响因子：
3.1
通讯作者：
Qinjun Wei;Wei He;Jun Yao;Li Guo;Ya-jie Lu;Xin Cao
中科院分区：
文献类型：
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作者：
Qinjun Wei;Wei He;Jun Yao;Li Guo;Ya-jie Lu;Xin Cao
microRNAs（miRNAs）是基因表达的关键调节因子，在多种生物学过程中发挥着重要作用。为了研究miRNAs在乳腺癌催乳素受体（prolactin receptor，PRLR）信号通路中的功能作用，我们构建了两个来自人乳腺癌T-47 D细胞的小RNA文库。采用Solexa深度测序技术系统筛选miRNA表达谱。超过40种miRNA显着差异表达，从中选择4种miRNA进行茎环实时PCR验证。此外，还筛选了3个新的miRNAs进行PCR验证。此外，采用不同的算法预测了上游miRNA靶基因，GO和KEGG分析显示这些靶基因与PRLR信号通路高度相关。本研究为阐明影响乳腺癌发生发展的PRL/PRLR信号通路的复杂miRNA调控网络提供了参考。
MicroRNAs (miRNAs) are key regulators of gene expression and perform critical roles in various biological processes. To investigate the functional roles of miRNAs in the prolactin receptor (PRLR) signaling pathway in breast cancer, we constructed two small RNA libraries from human breast cancer T-47D cells treated with or without prolactin (PRL). The miRNA expression profiles were systematically screened using Solexa deep-sequencing technology. More than 40 miRNAs were significantly differentially expressed, from which 4 miRNAs were chosen for validation by stem-loop real-time PCR. In addition, 3 novel miRNAs were selected for verification by PCR. Furthermore, upstream miRNA target genes were predicted using different algorithms, GO and KEGG analyses revealed that these targets were highly related to the PRLR signaling pathway. This study provides a reference for elucidating the complex miRNA-mediated regulatory networks of PRL/PRLR signaling pathway that affect breast cancer tumorigenesis and progression.