Chromosome Segment Substitution Lines: A Powerful Tool for the Introgression of Valuable Genes from Oryza Wild Species into Cultivated Rice (O. sativa)
Chromosome Segment Substitution Lines: A Powerful Tool for the Introgression of Valuable Genes from Oryza Wild Species into Cultivated Rice (O. sativa)
复制标题
染色体片段替换系：将野生稻中有价值的基因渗入栽培稻 (O. sativa) 的有力工具
DOI：
10.1007/s12284-010-9058-3
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发表时间：
2010-12-01
期刊：
影响因子：
5.5
通讯作者：
Eizenga, Georgia C.
中科院分区：
文献类型：
--
作者：
Ali, M. Liakat;Sanchez, Paul L.;Eizenga, Georgia C.
水稻（Oryza）属的野生种含有许多有用的基因，但这些基因中的绝大多数至今仍未被利用，因为通常难以将这些基因转移到栽培水稻（Oryza sativa L.）中。染色体片段代换系（CSSLs）和回交自交系（BILs）是在不适应种质中鉴定这些天然存在的有利等位基因的有力工具。在本文中，我们提出了一个概述的研究涉及CSSLs和BILs的数量性状基因座（QTL）的渗入与水稻的性能改善，包括各种生物和非生物胁迫的抗性，甚至高产量从野生近缘水稻和其他不适应种质适应水稻品种的遗传背景。CSSLs可用于将数量性状分解为组成遗传因子，并作为单因子（单基因位点）评估基因作用。CSSLs有潜力从不适应的、非生产性的野生稻种质中发现新的等位基因，开发全基因组遗传资源，并克隆QTL研究中鉴定的基因用于功能基因组学研究。最近开发的高密度单核苷酸多态性（SNP）阵列在水稻和定制设计的中，低密度SNP阵列的可用性将提高CSSL的发展过程与较小的标记定义的片段渐渗从不适应的种质。
Wild species of rice (genusOryza) contain many useful genes but a vast majority of these genes remain untapped to date because it is often difficult to transfer these genes into cultivated rice (Oryza sativaL.). Chromosome segment substitution lines (CSSLs) and backcross inbred lines (BILs) are powerful tools for identifying these naturally occurring, favorable alleles in unadapted germplasm. In this paper, we present an overview of the research involving CSSLs and BILs in the introgression of quantitative trait loci (QTLs) associated with the improved performance of rice including resistance to various biotic and abiotic stresses, and even high yield from wild relatives of rice and other unadapted germplasm into the genetic background of adapted rice cultivars. The CSSLs can be used to dissect quantitative traits into the component genetic factors and evaluate gene action as single factors (monogenic loci). CSSLs have the potential to uncover new alleles from the unadapted, non-productive wild rice accessions, develop genome-wide genetic stocks, and clone genes identified in QTL studies for functional genomics research. Recent development of high-density single-nucleotide polymorphism (SNP) arrays in rice and availability of custom-designed medium- and low-density SNP arrays will enhance the CSSL development process with smaller marker-defined segment introgressions from unadapted germplasm.