Changes in the localization of antigen presenting cells and T cells in the utero-vaginal junction after repeated artificial insemination in laying hens
Changes in the localization of antigen presenting cells and T cells in the utero-vaginal junction after repeated artificial insemination in laying hens
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DOI：
10.1262/jrd.17027
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发表时间：
2005-10-01
影响因子：
1.8
通讯作者：
Yoshimura, Y
中科院分区：
文献类型：
--
作者：
Das, SC;Nagasaka, N;Yoshimura, Y
我们本研究的目的是观察罗德岛红蛋鸡子宫阴道交界处（UVJ）的抗原呈递细胞（Ia+细胞）和T细胞亚群（CD4+和CD8+ T细胞）群体是否发生变化，这些鸡在多次人工授精（AI）后生育力急剧下降。将罗得岛州红蛋鸡分为两组：处女组（R-V）和人工授精组（R-AI），人工授精组每周暴露于人工授精中，为期3个月。人工授精使用从高知县饲养的日本本土品种Tosa-Jidori健康公鸡采集的未稀释的新鲜精液。对UVJ组织进行冷冻切片处理，免疫组化检测Ia+细胞、CD4+和CD8+ T细胞。在R-AI和R-V鸟的UVJ间质和粘膜上皮中均可见Ia+细胞、CD4+和CD8+ T细胞。它们在基质中的频率在R-AI中明显高于R-V。R-AI组UVJ中Ia+细胞的频率较高，表明其对CD4+细胞的抗原呈递能力更强。R-AI鸟类UVJ中CD8+和CD4+ T细胞的显著增加可能是淋巴细胞归巢过程的结果，这可能影响精子的生存能力和生育能力。
The goal of our present study was to observe whether the populations of antigen presenting cells (Ia+ cells) and T cell subsets (CD4+ and CD8+ T cells) change in the utero-vaginal junction (UVJ) of Rhode Island Red laying hens that showed dramatic declines in fertility after repeated artificial insemination (AI). Rhode Island Red laying hens were divided into two groups: a virgin group (R-V) and artificial inseminated group (R-AI), which was exposed to weekly AI for a period of 3 mo. Undiluted fresh semen collected from healthy Tosa-Jidori roosters, a native Japanese breed maintained in Kochi Prefecture, was used for AI. The UVJ tissues were processed for frozen sections, and Ia+ cells and CD4+ and CD8+ T cells were identified by immunohistochemistry. The Ia+ cells and CD4+ and CD8+ T cells were observed in the stroma and mucosal epithelium of UVJ in both the R-AI and R-V birds. The frequencies of them in the stroma were significantly higher in R-AI than R-V. The higher frequency of Ia+ cells in the UVJ of R-AI group indicated a greater potential capability for antigen presentation to CD4+ cells. The significant increase in CD8+ and CD4+ T cells in the UVJ of R-AI birds might be the result of a homing process of lymphocytes, which may affect sperm survivability and fertility.