Supergroup C Wolbachia, mutualist symbionts of filarial nematodes, have a distinct genome structure.
Supergroup C Wolbachia, mutualist symbionts of filarial nematodes, have a distinct genome structure.
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DOI：
10.1098/rsob.150099
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发表时间：
2015-12
期刊：
影响因子：
5.8
通讯作者：
Sassera D
中科院分区：
文献类型：
--
作者：
Comandatore F;Cordaux R;Bandi C;Blaxter M;Darby A;Makepeace BL;Montagna M;Sassera D
Wolbachia pipientis 可能是节肢动物和线虫中最广泛的内共生体。虽然所有沃尔巴克氏体菌株在历史上都被定义为单一物种，但已描述了 16 个单系多样性簇（称为超群）。不同的超级群体具有不同的宿主范围和共生关系，从互利共生到生殖操纵。丝虫线虫包括导致人类主要疾病（如盘尾丝虫，河盲症病原体）和伴侣动物（恶丝虫，狗恶丝虫）的寄生虫，沃尔巴克氏体具有专性共生作用，是新治疗方案的目标。在这里，我们比较了八个沃尔巴克氏体菌株的基因组，涵盖主要超群 (A-F) 的多样性，分析同线性、转座元件含量、GC 偏差和基因丢失或增益。我们检测到沃尔巴克氏体超群之间存在差异的基因组特征，最显着的是丝虫线虫的 C 和 D 分支。特别是，来自 C 超群的菌株（盘旋丝虫和粗丝线虫的共生体）呈现出 GC 倾斜、保守同线性和缺乏转座元件的模式，这在沃尔巴克氏体属中是独一无二的。这些特征可能是沃尔巴克氏体菌株与其宿主之间明显共生关系的结果，突显了丝虫线虫内发现的互利超群之间未被充分认识的差异。
Wolbachia pipientis is possibly the most widespread endosymbiont of arthropods and nematodes. While all Wolbachia strains have historically been defined as a single species, 16 monophyletic clusters of diversity (called supergroups) have been described. Different supergroups have distinct host ranges and symbiotic relationships, ranging from mutualism to reproductive manipulation. In filarial nematodes, which include parasites responsible for major diseases of humans (such as Onchocerca volvulus, agent of river blindness) and companion animals (Dirofilaria immitis, the dog heartworm), Wolbachia has an obligate mutualist role and is the target of new treatment regimens. Here, we compare the genomes of eight Wolbachia strains, spanning the diversity of the major supergroups (A–F), analysing synteny, transposable element content, GC skew and gene loss or gain. We detected genomic features that differ between Wolbachia supergroups, most notably in the C and D clades from filarial nematodes. In particular, strains from supergroup C (symbionts of O. volvulus and D. immitis) present a pattern of GC skew, conserved synteny and lack of transposable elements, unique in the Wolbachia genus. These features could be the consequence of a distinct symbiotic relationship between C Wolbachia strains and their hosts, highlighting underappreciated differences between the mutualistic supergroups found within filarial nematodes.