Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures
Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures
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DOI：
10.1515/bc.2004.027
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发表时间：
2004-03-01
影响因子：
3.7
通讯作者：
Abdel-Wahab, YHA
中科院分区：
文献类型：
--
作者：
Marenah, L;Flatt, PR;Abdel-Wahab, YHA
评价了蟾蜍铃蟾皮肤分泌物中促胰岛素肽的分离和表征。通过温和电刺激蟾蜍背部皮肤表面获得的粗分泌物经反相HPLC纯化，得到44个峰。在与葡萄糖响应性BRINBD 11细胞的急性孵育中，峰21、22、23、24和25显示与单独的5.6 mM葡萄糖相比胰岛素释放增加1.5-3.5倍（p
Skin secretions of the toad Bombina variegata were evaluated for the isolation and characterisation of insulinotropic peptides. Crude secretions obtained from young adult toads by mild electrical stimulation of the dorsal skin surface were purified by reverse phase HPLC yielding 44 peaks. In acute incubations with glucoseresponsive BRINBD11 cells, peaks 21, 22, 23, 24 and 25 showed a 1.5-3.5-fold increase in insulin release compared with 5.6 mM glucose alone (p