Epimedium koreanum Nakai and its main constituent icariin suppress lipid accumulation during adipocyte differentiation of 3T3-L1 preadipocytes
Epimedium koreanum Nakai and its main constituent icariin suppress lipid accumulation during adipocyte differentiation of 3T3-L1 preadipocytes
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DOI：
10.1016/s1875-5364(16)30079-6
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发表时间：
2016-09-01
影响因子：
4.6
通讯作者：
Jung Hyo-Won
中科院分区：
文献类型：
--
作者：
Han Yunk-Yung;Song Mi-Young;Jung Hyo-Won
肥胖与一些代谢异常有关，如2型糖尿病，并已成为全球主要的健康问题。本研究探讨了朝鲜淫羊藿及其主要成分淫羊藿苷对3T3-L1前脂肪细胞分化的影响。何首乌提取物和淫羊藿苷可显著减少3T3-L1脂肪细胞的脂质蓄积，并抑制PPARγ、C/EBPα和SREBP-1c的表达。它们还抑制脂肪酸合成酶(FAS)、酰基辅酶A合成酶(ACS1)和Perilipin。此外，HE提取物和淫羊藿苷显著增加AMPK的磷酸化。提示HE提取物和淫羊藿苷通过下调成脂转录因子抑制脂肪细胞分化，提示含淫羊藿苷的HE有可能作为一种潜在的治疗药物用于肥胖的治疗和预防。
Obesity is associated with a number of metabolic abnormalities such as type 2 diabetes and has become a major health problem worldwide. In the present study, we investigated the effects of Epimedium koreanum Nakai (Herba Epimedii, HE) and its main constituent icariin on the adipocyte differentiation in 3T3-L1 preadipocytes. HE extract and icariin significantly reduced lipid accumulation and suppressed the expressions of PPAR gamma, C/EBP alpha, and SREBP-1c in 3T3-L1 adipocytes. They also inhibited fatty acid synthase (FAS), acyl-Co A synthase (ACS1), and perilipin. Moreover, HE extract and icariin markedly increased the phosphorylation of AMPK. These results indicated that HE extract and icariin can inhibit the adipocyte differentiation through downregulation of the adipogenic transcription factors, suggesting that HE containing icariin may be used as a potential therapeutic agent in the treatment and prevention of obesity.