A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability
A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability
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DOI：
10.1007/s001220050007
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发表时间：
2000-01-01
影响因子：
5.4
通讯作者：
Clark, LJ
中科院分区：
文献类型：
--
作者：
Price, AH;Steele, KA;Clark, LJ
利用早先定位的抗旱旱稻品种巴拉和Azucena的F-2组合，构建了一个F-6重组自交系群体的RFLP和AFLP联合连锁图谱。该图谱包含101个RFLP标记和34个AFLP标记，位于17个连锁群上，覆盖1680 cM。还给出了另外4个RFLP标记和75个AFLP标记的大致定位位置，这些标记要么不能在图谱上获得唯一的位置，要么现有数据不足以进行可靠的定位，以及与根系穿透能力相关的性状的数量性状基因座(QTL)定位的结果。通过计算穿透由80%蜡和20%白色软石蜡组成的3 mm厚的层的根轴的数量来评估牙根渗透。在土壤强度较高的地方，良好的根系穿透性有望提高抗旱性。单标记分析发现了7个控制穿透蜡层根数的QTL。在相同的位置上，有7个QTL控制着穿透根数与总根数的比率。Bala阳性等位基因的超亲遗传解释了其中4个QTL。将这些QTL与以前报道的影响根形态的QTL进行比较，结果表明，提高根穿透能力的等位基因也可能使根更长或更粗。
A combined RFLP and AFLP linkage map of an F-6 recombinant inbred population, which was derived from a previously mapped F-2 of a cross between the two drought resistant upland rice varieties Bala and Azucena, is presented. The map contains 101 RFLP and 34 AFLP markers on 17 linkage groups covering 1680 cM. Also presented is the approximate mapping position of a further four RFLP and 75 AFLP markers, which either could not be given a unique place on the map or for which the available data is not sufficient to allow confident positioning, and the result of quantitative trait locus (QTL) mapping of traits related to root-penetration ability. Root penetration was assessed by counting the number of root axes that penetrated a 3 mm-thick layer consisting of 80% wax and 20% white soft paraffin. Good root penetration would be expected to increase drought resistance where soil strength is high. Single-marker analysis revealed seven QTLs for the number of roots which penetrate the wax layer. In identical locations were seven QTLs for the ratio of penetrated to the total number of roots. Transgressive inheritance of positive alleles from Bala explained four of these QTLs. Comparison of the QTLs identified here with previous reports of QTLs for root morphology suggest that alleles which improve root penetration ability may also either make the roots longer or thicker.