Noxa is necessary for hydrogen peroxide-induced caspase-dependent cell death
Noxa is necessary for hydrogen peroxide-induced caspase-dependent cell death
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DOI：
10.1016/j.febslet.2010.01.026
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发表时间：
2010-02-19
期刊：
影响因子：
3.5
通讯作者：
Tsujimoto, Yoshihide
中科院分区：
文献类型：
--
作者：
Aikawa, Tomonori;Shinzawa, Koei;Tsujimoto, Yoshihide
氧化应激诱导许多类型的细胞凋亡或坏死，这可引起组织损伤。过氧化氢（H2 O2）诱导Jurkat细胞凋亡。这种作用被抑制过表达的人Bcl-2，细胞色素c的沉默，并通过消融Bax/巴克，表明过氧化氢诱导的细胞凋亡介导的线粒体途径在Jurkat细胞。H2 O2处理引起Noxa蛋白的增加，通过激活转录因子4依赖的Noxa mRNA的积累和抑制Noxa蛋白的降解。H2 O2诱导的细胞凋亡受到Noxa沉默的强烈抑制，表明Noxa在这种形式的细胞凋亡中起着至关重要的作用。
Oxidative stress induces apoptosis or necrosis of many cell types, which can cause tissue injury. Hydrogen peroxide (H2O2) induced apoptotic death of Jurkat cells. This effect was inhibited by overexpression of human Bcl-2, by silencing of cytochrome c, and by ablation of Bax/Bak, indicating that H2O2-induced apoptosis was mediated by the mitochondrial pathway in Jurkat cells. Treatment with H2O2 caused an increase of Noxa protein, via activating transcription factor 4-dependent accumulation of Noxa mRNA and inhibition of Noxa protein degradation. H2O2-induced apoptosis was strongly suppressed by silencing of Noxa, indicating that Noxa plays a crucial role in this form of apoptosis.