Molecular characterization of dsRNA segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, W370, of the plant pathogen Rosellinia necatrix.
Molecular characterization of dsRNA segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, W370, of the plant pathogen Rosellinia necatrix.
复制标题
DOI：
10.1099/vir.0.19098-0
复制
发表时间：
2003-09
期刊：
影响因子：
--
通讯作者：
C. Wei;H. Osaki;T. Iwanami;N. Matsumoto;Y. Ohtsu
中科院分区：
文献类型：
--
作者：
C. Wei;H. Osaki;T. Iwanami;N. Matsumoto;Y. Ohtsu
白根腐病真菌Rosellinia Necatrix的一个弱毒力分离物W370此前已被证明含有12个dsRNA片段。在本研究中，我们测定了W370 dsRNAs第2和第5段的完整核苷酸序列。基因组片段2的核苷酸序列全长3773bp，有一个长的开放阅读框，编码1226个氨基酸残基，预测分子质量约为138.5 kDa。第5片段的核苷酸序列全长2089个碱基，有一个长的开放阅读框架，其推导的多肽含有646个氨基酸残基，预测的相对分子质量约为72 kDa。比较分析表明，推导出的蛋白片段2与科罗拉多硬壳热病毒(CTFV)和欧洲眼镜蛇病毒(EYAV)的VP2蛋白序列具有较高的同源性，而推导出的片段5蛋白序列与其他病毒蛋白没有同源性。从分离株W370的菌丝组织中观察到直径约80 nm的双壳球形颗粒与W370 dsRNAs相关。结果表明，与W370 dsRNA相关的病毒是呼肠孤病毒科的一种新呼肠孤病毒。该病毒被命名为Rosellinia抗腐烂病毒(RArV)。
A hypovirulent isolate, W370, of the white root rot fungus Rosellinia necatrix has previously been shown to harbour 12 dsRNA segments. In this study, complete nucleotide sequences of segments 2 and 5 of W370 dsRNAs were determined. The nucleotide sequence of genome segment 2 was 3773 bases long with a single long open reading frame (ORF) encoding 1226 amino acid residues with a predicted molecular mass of approximately 138.5 kDa. The nucleotide sequence of segment 5 was 2089 bases long with a single long ORF, whose deduced polypeptide contained 646 amino acid residues with a predicted molecular mass of about 72 kDa. Comparative analysis showed that the deduced protein sequence of segment 2 had significant homology with the putative VP2 of Colorado tick fever virus (CTFV) and European Eyach virus (EYAV) in the genus Coltivirus, but the deduced protein sequence of segment 5 had no similarity with other virus proteins. Double-shelled spherical particles approximately 80 nm in diameter associated with W370 dsRNAs were observed in a preparation from the mycelial tissue of isolate W370. The results demonstrated that the virus associated with W370 dsRNAs is a novel reovirus of the family Reoviridae. The virus was named Rosellinia anti-rot virus (RArV).