Molecular and phylogenetic analyses of bovine rhinovirus type 2 shows it is closely related to foot-and-mouth disease virus
Molecular and phylogenetic analyses of bovine rhinovirus type 2 shows it is closely related to foot-and-mouth disease virus
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DOI：
10.1016/j.virol.2007.12.019
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发表时间：
2008-04-10
期刊：
影响因子：
3.7
通讯作者：
Rieder, Elizabeth
中科院分区：
文献类型：
--
作者：
Hollister, Jason R.;Vagnozzi, Ariel;Rieder, Elizabeth
牛鼻病毒 2 (BRV2) 是牛呼吸道疾病的病原体，暂时归属于小核糖核酸病毒科鼻病毒属。克隆了 BRV2 基因组的近全长 cDNA，并确定了核苷酸序列。 BRV2 拥有一个假定的前导蛋白酶、一个小 2A 蛋白和一个 Poly(C) 束，这些都是口疮病毒的特征。 BRV-2 和 FMDV 多蛋白的比对显示 PI 区内 41% 的氨基酸是相同的。此外，2A、2C、3B(3)、3C 和 3D 蛋白质的相似度分别高达 67%、52%、52%、50% 和 64%。 BRV2 前导蛋白从病毒多蛋白中快速释放，并以与 FMDV 前导蛋白酶相似的速度裂解 eIF4G，这表明这些病毒中前导蛋白之间存在功能关系。结果表明，BRV2 与口蹄疫病毒密切相关，因此应被视为口疮病毒属中的一个新物种。 (C) 2007 Elsevier Inc. 保留所有权利。
Bovine rhinovirus 2 (BRV2), a causative agent of respiratory disease in cattle, is tentatively assigned to the genus Rhinovirus in the family Picornaviridae. A nearly full-length cDNA of the BRV2 genome was cloned and the nucleotide sequence determined. BRV2 possesses a putative leader proteinase, a small 2A protein and a poly(C) tract, which are characteristic of aphthoviruses. Alignment of BRV-2 and FMDV polyproteins showed that 41% of amino acids were identical within the PI region. Furthermore, 2A, 2C, 3B(3), 3C and 3D proteins are as much as 67%, 52%, 52%, 50%, and 64% identical, respectively. BRV2 leader protein is rapidly released from the viral polyprotein and cleaves eIF4G at a rate similar to FMDV leader protemase, suggesting a functional relationship between the leader protein in these viruses. The results suggest that BRV2 is closely related to FMDV and should therefore be considered as a new species within the genus Aphthovirus. (C) 2007 Elsevier Inc. All rights reserved.