Determination of ascorbic acid in individual rat hepatocyte by capillary electrophoresis with electrochemical detection
Determination of ascorbic acid in individual rat hepatocyte by capillary electrophoresis with electrochemical detection
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DOI：
10.1016/j.jchromb.2008.05.035
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发表时间：
2008-07-01
影响因子：
3
通讯作者：
Zhang, Shusheng
中科院分区：
文献类型：
--
作者：
Sun, Xuemei;Niu, Yan;Zhang, Shusheng
A method for the direct determination of ascorbic acid (AA) in individual rat hepatocyte based on capillary electrophoresis (CE) coupled with electrochemical detection (ECD) using a new kind of homemade carbon fiber micro-disk bundle electrode has been described. Individual rat hepatocytes were injected into a fused-silica capillary with an inner diameter of 25 mu m, and lysed by 0.1% sodium dodecylsulfate (SDS) as cell lysis solution. The following conditions were suitable for the determination of AA: running buffer, 1.83 x 10(-2) mol/l Na2HPO4-1.70 x 10(-3) mol/l NaH2PO4 (pH 7.8); separation voltage, 20.0 kV; detection potential, 0.80V (vs. saturated calomel electrode (SCE)). The concentration limit of detection (LOD) of the method was 1.7 x 10(-6) mol/l at a signal-to-noise (S/N) ratio of 3, and the mass LOD was 3.0 fmol. The linear dynamic range was from 5.0 x 10(-6) to 5.0 x 10(-4) Mol/l with a correlation coefficient of 0.9962 for the injection voltage of 5.0 kV and injection time of 10 s. The relative standard deviation (R.S.D.) was 0.85% for the migration time and 1.8% for the peak current. This method was successfully applied to AA determination in rat hepatocyte. The recovery was between 91% and 97%, and the amount of AA in single rat hepatocyte ranged from 28 to 63 fmol. (C) 2008 Elsevier B.V. All rights reserved.
