Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation
Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation
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DOI：
10.1016/j.femsle.2005.04.028
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发表时间：
2005-06-01
影响因子：
2.1
通讯作者：
Moriya, S
中科院分区：
文献类型：
--
作者：
Hayashi, M;Ogura, Y;Moriya, S
这里通过流式细胞术显示枯草芽孢杆菌YabA负调节复制起始的时间。当YabA水平降低时，复制开始于细胞质量降低，当水平升高时，启动延迟。复制起始的同步性也在低水平的YabA下被破坏。Yfp-YabA在细胞内定位为病灶。由于YabA被报道与DnaN（DNA聚合酶III的β亚基）相互作用，因此使用与DnaX（DNA聚合酶III的tau亚基）的Cfp融合来检查Yfp-YabA与聚合酶的共定位。据报道，YabA似乎仅在DNA复制的后期定位于复制叉。(C)2005年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.