Kinetics of plasma microRNA-499 expression in acute myocardial infarction
Kinetics of plasma microRNA-499 expression in acute myocardial infarction
复制标题
急性心肌梗死血浆 microRNA-499 表达的动力学。
DOI：
10.3978/j.issn.2072-1439.2014.11.32
复制
发表时间：
2015-05-01
影响因子：
2.5
通讯作者：
Han, Zhijun
中科院分区：
文献类型：
--
作者：
Chen, Xi;Zhang, Lizhu;Han, Zhijun
背景
据报道，微小RNA（miRNA）存在于人体血浆中，并且越来越多地被认为是疾病的生物标志物。本研究旨在探讨急性心肌梗死（AMI）患者心脏特异性microR-499（miR-499）的动力学。
方法
采用定量PCR方法检测了73例急性冠状动脉综合征（ACS）患者（包括53例AMI和20例不稳定型心绞痛（UA）患者）心肌组织中miR-499的循环浓度。30名健康志愿者作为对照。AMI组分别于入院后即刻、发病后12 h、24 h、3 d、7 d采血。UA组和健康对照组于入院后立即采集血浆标本。根据冠状动脉造影结果，采用Gensini评分评价冠状动脉狭窄程度和范围。
结果
53例AMI患者入院即刻miR-499表达水平显著高于20例UA患者和30例健康对照组（P<0.01）。在AMI患者中，在最后一次胸痛发作的24小时内观察到miR-499水平的可测量的增加，并且在7天后水平恢复到基线水平。AMI患者血浆miR-499水平与cTnI（r=0.384，P<0.01）和CK-MB（r=0.402，P<0.01）呈正相关。此外，AMI合并二支和三支冠状动脉病变（CAD）患者的miR-499水平显著高于单支CAD患者（P<0.05）。采用Gensini评分评价冠状动脉狭窄程度。miR-499与Gensini评分呈正相关（r=0.52，P<0.01）。AMI患者入院时miR-499水平显著高于PCI后24 h水平（P<0.01），且与LVEF呈负相关（r=0.36，P=0.008）。
结论
发现心脏特异性miRNA-499水平与心肌损伤成线性比例。miRNA-499有可能成为AMI的一个新的生物标志物，并可作为心肌缺血风险的预测因子。
BACKGROUND
MicroRNA (miRNA) is reported to be present in human plasma and has been increasingly suggested as a biomarker for diseases. Our study aimed to investigate the kinetics of cardiac-specific microR-499 (miR-499) in acute myocardial infarction (AMI).
METHODS
Circulating concentrations of cardiac enriched miR-499 were measured by quantitative PCR in 73 patients with acute coronary syndrome (ACS), including 53 with AMI and 20 with unstable angina (UA). Thirty healthy subjects were used as controls. Plasma samples in AMI group were obtained immediately after admission and at 12 h, 24 h, 3 d and 7 d after onset of symptoms. Plasma samples in UA and healthy control groups were collected immediately after admission. The severity and extent of coronary stenotic lesions were evaluated on the basis of coronary angiography using Gensini score.
RESULTS
miR-499 expression levels were significantly higher in the 53 AMI patients than in the 20 UA patients and 30 healthy controls immediately after admission (P<0.01). A measurable increase in miR-499 levels was observed in AMI patients within 24 h of the last onset of chest pain and the levels returned to the baseline after 7 d. Plasma miR-499 levels in the patients with AMI were positively-correlated with cTnI (r=0.384, P<0.01) and CK-MB (r=0.402, P<0.01). In addition, miR-499 levels in AMI patients with two- and three-vessel coronary artery disease (CAD) were significantly higher than those in patients with single-vessel CAD (P<0.05). Gensini scores were used to evaluate the severity of coronary stenosis. miR-499 were positively correlated with Gensini scores (r=0.52, P<0.01). miR-499 levels at admission were significantly higher than that those 24 h after percutaneous coronary intervention (PCI) in AMI patients (P<0.01) and were negatively correlated with LVEF (r=0.36, P=0.008).
CONCLUSIONS
Cardiac-specific miRNA-499 levels were found to be linearly proportional to myocardial damage. MiRNA-499 might prove to be a new biomarker for AMI and a predictor of the risk of myocardial ischemia.