A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing
A Strategy for Direct Mapping and Identification of Mutations by Whole-Genome Sequencing
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DOI：
10.1534/genetics.110.119230
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发表时间：
2010-09-01
期刊：
影响因子：
3.3
通讯作者：
Jarriault, Sophie
中科院分区：
文献类型：
--
作者：
Zuryn, Steven;Le Gras, Stephanie;Jarriault, Sophie
突变筛选已被证明对于无数生物过程的基因解析非常有效，但随后识别和分离致病突变通常是复杂且耗时的。我们通过建立一种新的策略使这个过程变得更加容易，该策略采用全基因组测序来同时绘制和识别突变，而不需要任何事先的遗传绘制。
Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.