From DNA sequence analysis to modeling replication in the human genome
From DNA sequence analysis to modeling replication in the human genome
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DOI：
10.1103/physrevlett.94.248103
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发表时间：
2005-06-24
影响因子：
8.6
通讯作者：
Arneodo, A
中科院分区：
文献类型：
--
作者：
Brodie, EB;Nicolay, S;Arneodo, A
我们探索了人类染色体上核苷酸组成链不对称的大规模行为。正如我们观察到的到目前为止实验确定的9个复制起点中的7个，(TA+GC)偏斜显示出相当剧烈的向上跳跃，在两个连续跳跃之间具有线性递减的轮廓。我们提出了一个复制模型，该模型具有位置良好的复制起点和随机终止，这解释了观察到的特征锯齿状倾斜轮廓。我们成功地鉴定了287对假定的相邻复制起点，其起点间距类似于1-2 MBP，很可能对应于在间期细胞核中观察到的复制焦点，并被认为是持续到后续细胞世代的稳定结构。
We explore the large-scale behavior of nucleotide compositional strand asymmetries along human chromosomes. As we observe for 7 of 9 origins of replication experimentally identified so far, the (TA+GC) skew displays rather sharp upward jumps, with a linear decreasing profile in between two successive jumps. We present a model of replication with well positioned replication origins and random terminations that accounts for the observed characteristic serrated skew profiles. We succeed in identifying 287 pairs of putative adjacent replication origins with an origin spacing similar to 1-2 Mbp that are likely to correspond to replication foci observed in interphase nuclei and recognized as stable structures that persist throughout subsequent cell generations.