Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.
Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.
复制标题
使用单克隆抗体表征神经嵴肿瘤中的神经生长因子受体。
DOI：
10.1073/pnas.81.21.6681
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发表时间：
1984
影响因子：
11.1
通讯作者：
Atkinson,B
中科院分区：
文献类型：
--
作者：
Ross,AH;Grob,P;Bothwell,M;Elder,DE;Ernst,CS;Marano,N;Ghrist,BF;Slemp,CC;Herlyn,M;Atkinson,B
通过使用一系列新的抗受体单克隆抗体 (MAb) 来表征神经生长因子 (NGF) 受体。这些 MAb (i) 与表达较高水平 NGF 受体的黑色素瘤细胞系表现出显着更高的反应性，(ii) 抑制 125I 标记的 NGF 与其受体的结合，以及 (iii) 免疫沉淀代谢标记的和 125I 标记的 NGF 亲和标记受体。这些实验将受体定义为 75 kDa 的细胞表面蛋白。通过免疫过氧化物酶染色，在人痣、黑色素瘤、神经纤维瘤、嗜铬细胞瘤和周围神经的组织切片中观察到 NGF 受体。细胞质的均匀染色表明，除了细胞表面 NGF 受体外，还存在一群细胞内受体。
The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors.
影响因子：
64.8
作者：
ALBINO, AP;LESTRANGE, R;OLD, LJ
通讯作者：
OLD, LJ