Two GLOBOSA-like genes are expressed in second and third whorls of homochlamydeous flowers in Asparagus officinalis L.
Two GLOBOSA-like genes are expressed in second and third whorls of homochlamydeous flowers in Asparagus officinalis L.
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DOI：
10.1093/pcp/pch040
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发表时间：
2004-03-01
影响因子：
4.9
通讯作者：
Kameya, T
中科院分区：
文献类型：
--
作者：
Park, JH;Ishikawa, Y;Kameya, T
花园芦笋 (Asparagus officinalis L.) 具有同质花。与百合和郁金香等百合科植物一样，芦笋的花被有两个几乎相同的花瓣状器官轮生，称为花被片。这些同质花的花结构可以用修改后的ABC模型来解释，其中B类基因的表达已扩展到轮生1，因此轮生1和轮生2的器官具有相同的花瓣状结构。在本研究中，我们从芦笋中分离和鉴定了两个 GLOBOSA 样基因（AOGLOA 和 AOGLOB），这是 B 类基因之一。 Southern印迹显示AOGLOA和AOGLOB基因是单拷贝基因。 Northern印迹分析表明这些基因在雄花和雌花中特异性表达。原位杂交结果表明，AOGLOA和AOGLOB基因的表达仅限于第2和第3轮（内花被片和雄蕊），在第1轮（外花被片）中未检测到。另一个芦笋 B 类基因 AODEF 也在外花被片中不表达 [Park 等人，2017]。 (2003)植物分子生物学。 51：867]。这些结果表明B类基因不参与芦笋的外花被发育，不支持芦笋中改良的ABC模型。
Garden asparagus (Asparagus officinalis L.) has homo- chlamydeous flowers. Like Liliaceae plants such as lily and tulip, the perianths of asparagus have two whorls of almost identical petaloid organs, called tepals. Floral structures of these homochlamydeous flowers could be explained by a modified ABC model, in which the expression of the class B genes has expanded to whorl 1, so that the organs of whorls I and 2 have the same petaloid structure. In this study, we isolated and characterized two GLOBOSA-like genes (AOGLOA and AOGLOB), one of class B gene, from asparagus. Southern blot showed that AOGLOA and AOGLOB genes are single copy genes. Northern blot analysis indicated that these genes were specifically expressed in male and female flowers. In situ hybridization showed that the expression of AOGLOA and AOGLOB genes is confined to whorls 2 and 3 (inner tepal and stamen) and not detected in whorl I (outer tepal). The other asparagus class B gene, AODEF, was also not expressed in outer tepal [Park et al. (2003) Plant Mol Biol. 51: 867]. These results indicate that the class B genes are not involved in the outer tepal development in asparagus, not supporting the modified ABC model in asparagus.