Oxidizing intermediates in the reaction of ferrous EDTA with hydrogen peroxide. Reactions with organic molecules and ferrocytochrome c.
Oxidizing intermediates in the reaction of ferrous EDTA with hydrogen peroxide. Reactions with organic molecules and ferrocytochrome c.
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DOI：
10.1016/s0021-9258(19)62677-3
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发表时间：
1986-05
期刊：
影响因子：
--
通讯作者：
J. D. Rush;Willem H. Koppenolg
中科院分区：
文献类型：
--
作者：
J. D. Rush;Willem H. Koppenolg
过氧化氢与亚铁EDTA反应生成一种氧化中间体(I1)，它不是羟基自由基。它氧化细胞色素c，并与过氧化氢(K_5=3.2×10~(-3)M~(-1)S~(-1))反应生成第二个氧化瞬变(I_2)。I_1不被叔丁醇清除，而I_2被叔丁醇清除。研究发现，I1对苯甲酸根、叔丁醇、醋酸根、精氨酸和丝氨酸的活性明显低于羟基自由基，但可被乙醇和异丙醇等易氧化官能团的化合物清除。这表明I1不经历羟基自由基的特征反应，但显示出一种与金属离子氧化剂更相关的反应模式，如铁基(FeO2+)-EDTA络合物。
The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-butyl alcohol whereas I2 is. I1 is found to be significantly less reactive than the hydroxyl radical toward benzoate ion, t-butyl alcohol, acetate ion, arginine, and serine, but is scavenged by compounds with readily oxidizable functional groups such as ethanol and isopropyl alcohol. This indicates that I1 does not undergo the characteristic reactions of the hydroxyl radical but shows a pattern of reactivity more associated with a metal ion oxidant like a ferryl (FeO2+)-EDTA complex.