Perfluorochemical emulsions decrease Kupffer cell phagocytosis.
Perfluorochemical emulsions decrease Kupffer cell phagocytosis.
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全氟化合物乳液会降低库普弗细胞的吞噬作用。
DOI：
10.1002/hep.1840140127
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Weinstock,SB
中科院分区：
文献类型：
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作者：
Bottalico,LA;Betensky,HT;Min,YB;Weinstock,SB
使用全氟化学乳剂作为血液替代品的一个缺点是，全氟化学颗粒被网状内皮系统(主要是肝和脾)从血液中清除。我们测定了两种全氟化学乳剂对体内网状内皮系统清除胶体碳(~lt；1μm)和51铬绵羊红细胞(约8μm)的影响。雄性大鼠连续4天注射2ml/100gg体重的氟酚-DA或氧化苦参素-ET。然后将碳(1毫升/100克体重)或绵羊红细胞(0.05毫升5%VOL/100克体重)静脉注射(体内)或添加到灌流液中。在几个时间点采集样本，持续1小时。在离体灌流肝中，治疗后第1天碳廓清降低了25%。在氟化多巴胺处理的大鼠中，12天后比率恢复到对照水平，但在氧化苦参素-ET处理的大鼠中，比率仍然保持在67%的低水平。绵羊红细胞(8μm)清除速度比碳清除慢2~5倍，给药后1天和12天，大鼠肝脏中的碳清除速度分别降低40%。添加血清并不能改善吞噬功能。在体内，氟-DA组大鼠的碳廓清仍保持正常，但氧化苦参素-ET组的大鼠在治疗后1天降低了74%，12天后恢复正常。体内对照大鼠和灌流肝脏中的清除率相似。结论：离体肝灌流模型是衡量肝脏清除功能的良好模型。虽然低剂量的全氟化学乳剂可能会抑制库普弗细胞的吞噬功能，但一般网状内皮系统的功能并未受到显著影响(肝病1991；14：169-174)。
One drawback to using perfluorochemical emulsions as blood substitutes is that perfluorochemical particles are cleared from the blood by the reticuloendothelial system, primarily liver and spleen. We measured the impact of two perfluorochemical emulsions on clearance of colloidal carbon (< 1 μm) and 51 Cr-sheep red blood cells (about 8 μm) by the reticuloendothelial system in vivo and in the isolated perfused liver. Male rats were injected with 2 ml/100 gm body wt of Fluosol-DA or Oxypherol-ET for 4 consecutive days. Carbon (1 ml/100 gm body wt) or sheep red blood cells (0.05 ml of 5% vol/vol/100 gm body wt) were then injected intravenously (in vivo) or added to perfusate. Samples were taken at several time points for 1 hr. In the isolated perfused liver, carbon clearance was depressed by 25% 1 day after treatment. Rates returned to control levels by 12 days in Fluosol-DA—treated rats but remained depressed by 67% in Oxypherol-ET—treated rats. Sheep red blood cell (8 μm) clearance was two to five times slower than carbon clearance and depressed by 40% in livers from Fluosol-DA rats 1 day and 12 days after treatment. Added serum did not improve phagocytosis. In vivo carbon clearance remained normal in Fluosol-DA—treated rats but decreased by 74% in Oxypherol-ET—treated rats 1 day after treatment, returning to normal by 12 days. Clearance rates were similar in control rats in vivo and in the perfused liver. We conclude that the isolated perfused liver is a good model to measure liver clearance function. Although low doses of perfluorochemical emulsions may depress Kupffer cell phagocytosis, general reticuloendothelial system function is not significantly compromised.(HEPATOLOGY 1991; 14: 169-174.)