Potassium effects on bone: comparison of two model systems.
Potassium effects on bone: comparison of two model systems.
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钾对骨骼的影响：两种模型系统的比较。
DOI：
10.1152/ajpendo.1983.245.3.e303
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Stern,PH
中科院分区：
文献类型：
--
作者：
Krieger,NS;Stern,PH
在新生小鼠颅骨、胎鼠肢骨和胎鼠肢骨中研究了钾升高对骨吸收的影响以及哇巴因对甲状旁腺激素（PTH）刺激的骨吸收的抑制作用。哇巴因抑制PTH刺激的吸收，和K至少部分逆转哇巴因在所有三个系统中的抑制。然而，与颅盖骨相反，增加K对两肢骨系统均无再吸收刺激。虽然哇巴因抑制在所有三个系统中的逆转可能是通过对Na-K-ATP酶的作用介导的，但单独K的再吸收作用必须通过不同的机制发生，因为它仅在颅骨中观察到。在K刺激钙释放的机制中，野牡丹素的产生可能起部分作用。钾（35 mM）刺激PGE 2的生产由颅盖骨，但不是四肢骨。吲哚美辛可抑制颅骨中PGE 2的增加，并部分阻断K.胎鼠肢体骨培养物与小鼠颅骨的不同之处在于更容易受到渗透压增加的抑制。因此，继发效应可能是不同骨组织对某些刺激的不同反应的原因。
Effects of elevated potassium on bone resorption and on the inhibition by ouabain of parathyroid hormone (PTH)-stimulated resorption were studied in neonatal mouse calvaria, fetal mouse limb bones, and fetal rat limb bones. Ouabain inhibited PTH-stimulated resorption, and K at least partially reversed the inhibition by ouabain in all three systems. However, in contrast to calvaria, neither limb bone system was stimulated to resorb by increased K. Although the reversal of ouabain inhibition in all three systems was likely mediated by an effect on Na-K-ATPase, the resorptive effect of K alone must occur by a different mechanism because it was seen only in the calvaria. The production of prostaglandins may play a partial role in the mechanism of the stimulation of Ca release from calvaria by K. Potassium (35 mM) stimulated production of PGE2 by calvaria but not by limb bones. Indomethacin inhibited the increase in PGE2 in calvaria and partially blocked the stimulated bone resorption observed in response to K. The fetal rat limb bone cultures also differed from the mouse calvaria in being more readily inhibited by increased osmolarity. Thus, secondary effects may be responsible for the variant responses of different bone tissues to certain stimuli.