EXPRESSION OF A SET OF GROWTH-RELATED IMMEDIATE EARLY GENES IN BALB-C 3T3 CELLS - COORDINATE REGULATION WITH C-FOS OR C-MYC
EXPRESSION OF A SET OF GROWTH-RELATED IMMEDIATE EARLY GENES IN BALB-C 3T3 CELLS - COORDINATE REGULATION WITH C-FOS OR C-MYC
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DOI：
10.1073/pnas.84.5.1182
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发表时间：
1987-03-01
影响因子：
11.1
通讯作者：
NATHANS, D
中科院分区：
文献类型：
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作者：
LAU, LF;NATHANS, D
我们之前通过 cDNA 克隆鉴定了 5 个 mRNA，这些 mRNA 在血清或血小板衍生生长因子生长刺激后不久出现在静息 BALB/c 3T3 细胞中。本报告描述了此类的另外 5 种 mRNA。添加血清后 40 至 120 分钟，mRNA 达到峰值水平，此后迅速衰减。所有 10 个 RNA 在放线菌酮存在下均被超诱导。核连续实验表明，mRNA 的增加是其基因在血清或血小板衍生生长因子刺激下快速转录激活的结果。放线菌酮的超诱导作用归因于两种效应：延长转录和稳定 mRNA。这种总体调控模式与之前报道的 c-fos 或 c-myc 原癌基因的调控模式相似。我们假设这些新发现的“立即早期”基因在生长因子诱导的增殖反应中发挥作用。
We have previously identified by cDNA cloning 5 mRNAs that appear in resting BALB/c 3T3 cells soon after growth stimulation by serum or platelet-derived growth factor. Five additional mRNAs of this class are described in this report. The mRNAs reached peak levels between 40 and 120 min after serum addition and rapidly decayed thereafter. All 10 RNAs were superinduced in the presence of cycloheximide. Nuclear run-on experiments indicated that the increase in the mRNAs is the result of rapid transcriptional activation of their genes on stimulation by serum or platelet-derived growth factor. Superinducibility by cycloheximide is due to two effects: prolonged transcription and stabilization of mRNAs. This overall pattern of regulation is similar to that of the c-fos or c-myc protooncogenes reported previously. We hypothesize that these newly identified "immediately early" genes play a role in the proliferative response induced by growth factors.