Interaction of human neutrophils with endothelial cells regulates the expression of endogenous proteins annexin 1, galectin-1 and galectin-3
Interaction of human neutrophils with endothelial cells regulates the expression of endogenous proteins annexin 1, galectin-1 and galectin-3
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DOI：
10.1016/j.cellbi.2005.12.010
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发表时间：
2006-04-01
影响因子：
3.9
通讯作者：
Oliani, SM
中科院分区：
文献类型：
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作者：
Gil, CD;La, M;Oliani, SM
膜联蛋白 1 (ANXA1)、半乳糖凝集素 1 (Gal-1) 和半乳糖凝集素 3 (Gal-3) 蛋白已被确定为促进或抑制白细胞迁移的重要介质。研究人员在 IL-8 诱导的迁移过程中研究了人类中性粒细胞和内皮细胞 (EC) 中这些蛋白质的表达。中性粒细胞粘附到 EC 后，与完整的 ANXA1 同工型（LCPS1，针对蛋白质的 N 末端）相比，在粘附的中性粒细胞和 EC 的质膜中检测到裂解的 ANXA1（LCS3，针对所有 ANXA1 亚型）表达显着增加。贴壁中性粒细胞的细胞核和细胞质中的 Gal-3 水平以及质膜中的 EC 水平升高。相反，与非迁移的中性粒细胞相比，在迁移的中性粒细胞中检测到 Gal-1 的总量减少。因此，当中性粒细胞粘附在内皮细胞上时，ANXA1和Gal-3的含量和定位发生变化，表明两种内源性抗炎和促炎介质之间存在敏感平衡的过程。 (c) 2006 年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Annexin 1 (ANXA1), galectin-1 (Gal-1) and galectin-3 (Gal-3) proteins have been identified as important mediators that promote or inhibit leukocyte migration. The expression of these proteins was studied in human neutrophils and endothelial cells (ECs) during a transmigration process induced by IL-8. Upon neutrophil adhesion to EC, a significant increase in the cleaved ANXA1 (LCS3, raised against all ANXA1 isoforms) expression was detected in the plasma membrane of adhered neutrophils and ECs compared to intact ANXA1 isoform (LCPS1, against N-terminus of protein). Adherent neutrophils had elevated Gal-3 levels in the nucleus and cytoplasm, and ECs in their plasma membranes. In contrast, a decrease in the total amounts of Gal-1 was detected in migrated compared to non-migrated neutrophils. Therefore, ANXA1 and Gal-3 changed in their content and localization when neutrophils adhere to endothelia, suggesting a process of sensitive-balance between two endogenous anti- and pro-inflammatory mediators. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.