TRAM-Derived Decoy Peptides inhibits the inflammatory response in mouse mammary epithelial cells and a mastitis model in mice
TRAM-Derived Decoy Peptides inhibits the inflammatory response in mouse mammary epithelial cells and a mastitis model in mice
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TRAM 衍生的诱饵肽可抑制小鼠乳腺上皮细胞和小鼠乳腺炎模型的炎症反应。
DOI：
10.1016/j.ejphar.2015.06.022
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发表时间：
2015-10-05
影响因子：
5
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
--
作者：
Hu, Xiaoyu;Tian, Yuan;Zhang, Naisheng
已经证明TRAM衍生的诱饵肽具有抗炎特性。本研究合成了TRAM TIR结构域的诱饵肽（TM 6），其序列为“N”-RQIKIWYNRRMKWK，KENFLRDTWCNYFY-“C”，并评价了TM 6对脂多糖诱导的小鼠乳腺炎的影响。在体内，通过乳腺导管注射LPS建立小鼠乳腺炎模型。在CPS治疗前或治疗后1 h注射TM 6。在体外，原代小鼠乳腺上皮细胞用于研究TM 6对LPS诱导的炎症反应的影响。结果表明，TM 6可抑制LPS诱导的小鼠乳腺组织病理学改变、MPO活性及TNF-α、IL-1 β和IL-6的产生。在体外，TM 6显著抑制LPS诱导的TNF-α和IL-6的产生，以及NF-κ B和MAPK的活化。总之，本研究表明TM 6对LPS-乳腺炎具有保护作用，可能是一种有前途的乳腺炎治疗剂。(C)2015 Elsevier B. V.版权所有。
It has been proved that TRAM-Derived Decoy peptides have anti-inflammatory properties. In this study, we synthesized a TRAM-Derived decoy peptide (TM6), belongs to TRAM TIR domain, of which sequence is "N"-RQIKIWFQNRRMKWK, KENFLRDTWCNFQFY-"C" and evaluated the effects of TM6 on lipopolysaccharide-induced mastitis in mice. In vivo, LPS-induced mice mastitis model was established by injection of LPS through the duct of mammary gland. TM6 was injected 1 h before or after CPS treatment. In vitro, primary mouse mammary epithelial cells were used to investigate the effects of TM6 on LPS induced inflammatory responses. The results showed that TM6 inhibited LPS induced mammary gland histopathologic changes, MPO activity, and TNF-alpha, IL-1 beta and IL-6 production in mice. In vitro, TM6 significantly inhibited LPS induced TNF-alpha and IL-6 production, as well as NF-kappa B and MAPKs activation. In conclusion, this study demonstrated that TM6 had protective effects on LPS-mastitis and may be a promising therapeutic reagent for mastitis treatment. (C) 2015 Elsevier B.V. All rights reserved.