Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean
Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean
复制标题
DOI：
10.1002/pmic.200900308
复制
发表时间：
2009-11-01
期刊：
影响因子：
3.4
通讯作者：
Komatsu, Setsuko
中科院分区：
文献类型：
--
作者：
Ahsan, Nagib;Komatsu, Setsuko
采用蛋白质组学方法研究了大豆不同发育阶段各器官和组织中蛋白质网络的功能分化。采用Mg/NP-40缓冲液提取蛋白质，再采用碱性苯酚法进行蛋白质组学分析。不同发育阶段叶片的蛋白质组学分析显示，26个蛋白存在差异表达，其中叶绿体蛋白转运机制外/内包膜转位蛋白在第一三叶显著增加。免疫印迹分析显示，chaperonin-60在幼叶中大量表达，而hsp70和atp合酶β在所有组织中均组成性表达。叶片净光合速率与叶绿素含量呈年龄相关。这些结果表明，参与碳同化、折叠和组装以及能量的蛋白质可能同步工作，并与叶片发育阶段的光合作用呈线性相关。花芽和花的蛋白质组比较发现29个差异表达蛋白，其中涉及线粒体蛋白运输和组装、次级代谢和花粉管生长的蛋白在花发育过程中上调。总之，这些结果表明，在发育阶段，每种类型的组织都与一组特定的蛋白质相关；其中，参与能量、糖代谢、折叠、组装和目的地的蛋白质可能在每个器官或组织的成熟过程中起关键作用。
The functional differentiation of protein networks in individual organs and tissues of soybean at various developmental stages was investigated by proteomic approach. Protein extraction by Mg/NP-40 buffer followed by alkaline phenol-based method was optimized for proteomic analysis. Proteome analyses of leaves at various developmental stages showed 26 differentially expressed proteins, wherein proteins in translocon at the outer/inner envelope membrane of chloroplast protein-transport machineries increased significantly at the first trifoliate. Immunoblot analysis showed chaperonin-60 expressed abundantly in young leaves, whereas HSP 70 and ATP-synthase beta were constitutively expressed in all tissues. The net photosynthesis rate and chlorophyll content showed an age-dependent correlation in leaves. These results suggest that proteins involved in carbon assimilation, folding and assembly, and energy may work synchronously and show a linear correlation to photosynthesis at developmental stages of leaves. Comparison of flower bud and flower proteome reveals 29 differentially expressed proteins, wherein proteins involved in mitochondrial protein transport and assembly, secondary metabolism, and pollen-tube growth were up-regulated during flower development. Together, these results suggest that during developmental stages, each type of tissue is associated with a specific group of proteins; wherein proteins involved in energy, sugar metabolism, and folding, assembly, and destination may play pivotal roles in the maturation process of each organ or tissue.