Transcriptomic Signatures of Alterations in a Myoblast Cell Line Infected with Four Distinct Strains of Trypanosoma cruzi
Transcriptomic Signatures of Alterations in a Myoblast Cell Line Infected with Four Distinct Strains of Trypanosoma cruzi
复制标题
DOI：
10.4269/ajtmh.2010.09-0399
复制
发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Spray, David C.
中科院分区：
文献类型：
--
作者：
Adesse, Daniel;Iacobas, Dumitru A.;Spray, David C.
我们检查了不同的克氏Thpanosoma cruzi菌株在感染巴西（TC I）、Y（TC II）、CL（IC II）和Tulahuen（TC II）菌株后72小时诱导培养的L6E9成肌细胞转录组变化的程度。在所有对照和感染 RNA 样本的四个副本中，使用 27,000 个大鼠寡核苷酸阵列对 6,289 个不同的、完全注释的 unigenes 的表达进行了定量。考虑到变化大于 1.5 倍且 P 值 < 0.05，Tulahuen 菌株对宿主转录组的破坏性最大（17% 的基因发生显着改变），而 Y 菌株仅改变了 6% 的基因。受感染细胞中显着改变的基因在菌株之间有很大差异，所有四种菌株中只有 21 个基因发生了类似的改变。然而，感染不同菌株的成肌细胞显示出成比例的整体基因表达改变。这些结果表明，不同寄生虫菌株的感染在宿主细胞中调节相似但不相同的途径。
We examined the extent to which different Thpanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (IC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.