Basic nanostructure of stratum corneum lipid matrices based on ceramides [EOS] and [AP]: a neutron diffraction study.
Basic nanostructure of stratum corneum lipid matrices based on ceramides [EOS] and [AP]: a neutron diffraction study.
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基于神经酰胺 [EOS] 和 [AP] 的角质层脂质基质的基本纳米结构：中子衍射研究
DOI：
10.1016/j.bpj.2009.05.041
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发表时间：
2009
影响因子：
3.4
通讯作者：
Neubert
中科院分区：
文献类型：
--
作者：
Schröter;Kessner;Kiselev;Neubert
本研究的目的是研究SC脂质模型膜的纳米结构，其包含最相关的SC脂质，例如具有接近自然比例的独特结构的ω-酰基神经酰胺[EOS]。在最近提出的模型中，神经酰胺[EOS]和FFA的存在对于形成SC脂质的两个存在的结晶层状相之一是必要的，的长周期性阶段，以及正常的屏障功能的SC。本研究的重点放在FFA BA的膜结构和其定位的膜内的神经酰胺[EOS]和[AP]的基础上的影响。这种膜的内部纳米结构通过傅立叶合成从实验衍射图案获得。由此产生的中子散射长度密度分布图显示，异常长的神经酰胺[EOS]被安排在神经酰胺[AP]通过跨越整个双层并进一步延伸到相邻双层中而产生的短周期性相中。特别是氘代BA使我们能够确定该FFA在该SC脂质模型膜内的确切位置。此外，水合实验表明，所提出的SC模拟系统显示出非常小的膜间水合，因此相邻小叶的头基彼此靠近。
The goal of this study was to investigate the nanostructure of SC lipid model membranes comprising the most relevant SC lipids such as the unique-structuredω-acylceramide [EOS] in a near natural ratio with neutron diffraction. In models proposed recently the presence of ceramide [EOS] and FFA are necessary for the formation of one of the two existent crystalline lamellar phases of the SC lipids, the long-periodicity phase as well as for the normal barrier function of the SC. The focus of this study was placed on the influence of the FFA BA on the membrane structure and its localization within the membrane based on the ceramides [EOS] and [AP]. The internal nanostructure of such membranes was obtained by Fourier synthesis from the experimental diffraction patterns. The resulting neutron scattering length density profiles showed that the exceptionally long ceramide [EOS] is arranged in a short-periodicity phase created by ceramide [AP] by spanning through the whole bilayer and extending even further into the adjacent bilayer. Specifically deuterated BA allowed us to determine the exact position of this FFA inside this SC lipid model membrane. Furthermore, hydration experiments showed that the presented SC mimic system shows an extremely small intermembrane hydration of ∼1 Å, consequently the headgroups of the neighboring leaflets are positioned close to each other.