Novel cancer suppressor gene for esophageal cancer: manganese superoxide dismutase
Novel cancer suppressor gene for esophageal cancer: manganese superoxide dismutase
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DOI：
10.1111/j.1442-2050.2010.01149.x
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发表时间：
2011-07-01
影响因子：
2.6
通讯作者：
Liu, Q.
中科院分区：
文献类型：
--
作者：
Sun, G. -G.;Wang, Y. -D.;Liu, Q.
目的：探讨锰超氧化物歧化酶（MnSOD）在食管鳞状细胞癌（ESCC）中的表达及其与临床病理特征和生物学行为的关系。对45例食管鳞癌患者进行免疫组化（SP法）、逆转录-聚合酶链反应（RT-PCR）、采用免疫印迹法检测食管鳞癌及癌旁组织中MnSOD蛋白和mRNA的表达。同时分析食管癌的病理学特征与其生物学行为的关系。食管鳞癌组织和正常食管组织中MnSOD蛋白阳性表达率分别为31.1%（14/45）和86.7%（31/45）MnSOD mRNA相对表达量分别为0.310 ± 0.036和0.482 ± 0.053（P = 0.000）。Westernblot结果显示MnSOD蛋白在癌组织和癌旁正常组织中的相对表达量分别为0.384 ± 0.038和0.766 ± 0.041（P = 0.000）。MnSOD蛋白和mRNA的表达水平与食管鳞癌的病变长度、浸润深度和分化程度密切相关（P < 0.05）。结论：食管鳞癌组织中MnSOD蛋白和mRNA表达均降低，可能与食管癌的发生、发展有关。因此，检测食管癌组织中MnSOD的表达对了解食管癌的生物学行为和指导食管癌的治疗策略具有重要的临床意义。
Objectives: To explore the expression of manganese superoxide dismutase (MnSOD) in esophageal squamous cell carcinoma (ESCC) and its relationship with clinicopathological characteristics and biological behavior.Methods: On 45 patients with ESCC, immunohistochemistry (SP method), reverse transcription-polymerase chain reaction (RT-PCR), and Western blot were used to detect MnSOD protein and mRNA expression in ESCC and in its adjacent normal tissue 5 cm apart from the edge of cancer lesion and without documented microscopic invasive cancer. Meanwhile, the relationship between the pathological features of esophageal cancer and its biological behavior was analyzed.Results: In ESCC and normal esophageal tissue, MnSOD protein expression was identified 31.1% (14/45) and 86.7% (31/45) (P = 0.000), respectively, with the relative expression levels of MnSOD mRNA 0.310 +/- 0.036 and 0.482 +/- 0.053 (P = 0.000), relatively. Western blot study showed that the relative expressions of MnSOD protein in cancer lesion and in adjacent normal tissue were 0.384 +/- 0.038 and 0.766 +/- 0.041, respectively (P = 0.000). With longer lesion, deeper invasion, and poorer differentiation, the expression of MnSOD would get lower, indicating that the levels of MnSOD protein and mRNA expression were closely related to the length of lesion, depth of invasion, and degree of differentiation in ESCC (P < 0.05). Nevertheless, the result showed no association with the presence of lymph node metastasis, cancer location, and macroscopic classification (P > 0.05).Conclusions: MnSOD protein and mRNA expression both decreased in ESCC, which may be related to carcinogenesis and the development of esophageal cancer. Therefore, detecting the expression of MnSOD in esophageal carcinoma would be of clinical significance in understanding its biological behavior and in guiding therapeutic strategy of esophageal cancer.