Epidemiological and genetic correlates of severe acute respiratory syndrome coronavirus infection in the hospital with the highest nosocomial infection rate in Taiwan in 2003
Epidemiological and genetic correlates of severe acute respiratory syndrome coronavirus infection in the hospital with the highest nosocomial infection rate in Taiwan in 2003
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DOI：
10.1128/jcm.44.2.359-365.2006
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发表时间：
2006-02-01
影响因子：
9.4
通讯作者：
Chu, DC
中科院分区：
文献类型：
--
作者：
Chen, YMA;Liang, SY;Chu, DC
台湾在2003年经历了一系列医院感染严重急性呼吸系统综合征（SARS）的爆发。最后一次爆发两个月后，我们从第一次也是最严重的SARS感染医院招募了658名员工，帮助我们调查与SARS冠状病毒（SARS-CoV）相关的流行病学和遗传因素。SARS-CoV感染是通过酶免疫分析检测的，并通过Western印迹分析、中和抗体测试和商业SARS测试的组合来确认。通过问卷调查和人类白细胞抗原（HLA）等位基因的序列特异性寡核苷酸探针分析危险因素。我们的研究结果表明，3%（20/658）的研究参与者血清阳性，一名女护士被确定为亚临床病例。确定的SARS-CoV感染风险因素包括与医院急诊室和感染病房在同一栋大楼工作，为SARS患者提供直接护理，以及携带Cw*0801 HLA等位基因。Cw*0801基因纯合子或杂合子感染SARS冠状病毒的优势比为4.4（95%可信区间为1.5 ~ 12.9，P = 0.007）。
Taiwan experienced a series of outbreaks of nosocomial severe acute respiratory syndrome (SARS) infections in 2003. Two months after the final outbreak, we recruited 658 employees from the hospital that suffered the first and most severe SARS infections to help us investigate epidemiological and genetic factors associated with the SARS coronavirus (SARS-CoV). SARS-CoV infections were detected by using enzyme immunoassays and confirmed by a combination of Western blot assays, neutralizing antibody tests, and commercial SARS tests. Risk factors were analyzed via questionnaire responses and sequence-specific oligonucleotide probes of human leukocyte antigen (HLA) alleles. Our results indicate that 3% (20/658) of the study participants were seropositive, with one female nurse identified as a subclinical case. Identified SARS-CoV infection risk factors include working in the same building as the hospital's emergency room and infection ward, providing direct care to SARS patients, and carrying a Cw*0801 HLA allele. The odds ratio for contracting a SARS-CoV infection among persons with either a homozygous or a heterozygous Cw*0801 genotype was 4.4 (95% confidence interval, 1.5 to 12.9; P = 0.007).