Optimal branched-chain amino acid ratio improves cell proliferation and protein metabolism of porcine enterocytesin in vivo and in vitro
Optimal branched-chain amino acid ratio improves cell proliferation and protein metabolism of porcine enterocytesin in vivo and in vitro
复制标题
最佳支链氨基酸比例可改善体内外猪肠上皮细胞的细胞增殖和蛋白质代谢
DOI：
10.1016/j.nut.2018.03.057
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发表时间：
2018-10-01
期刊：
影响因子：
4.4
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Duan, Yehui;Tan, Bie;Yin, Yulong
目的：研究不同支链氨基酸比例(1：1：1、1：0.75：0.75、1：0.51：0.63、1：0.25：0.25)对生长猪(平均初始体重9.85+/-0.35 kg)肠道细胞增殖和蛋白质代谢的影响。细胞增殖，氨基酸转运体基因表达。本实验以猪空肠上皮细胞为研究对象，研究不同支链氨基酸比例(0、1：0.25：0.25、1：0.5：0.5、1：1：1)对细胞增殖、AA转运蛋白表达的影响。结果：与阳性对照组(Leu：Ile：Val=1：0.51：0.63，20%CP)相比，1：0.75：0.75组(17%CP)空肠绒毛高度、增殖细胞核抗原阳性细胞百分率显著增加，回肠隐窝深度显著降低(P<；0.05)。此外，17%CP组仔猪空肠和回肠粘膜中的大部分AA浓度和AA转运体的表达水平均提高到与阳性对照组相近的水平。此外，体外研究还发现，1：0.25：0.25的支链氨基酸比例显著促进了肠道细胞的增殖、AA转运体的mRNA表达、AA浓度和蛋白质周转(P<；0.05)。结论：适宜的支链氨基酸比例(体内=1：0.75：0.75；体外=1：0.25：0.25)可以改善肠道形态和细胞增殖，通过调节肠道AA转运体的表达来增加肠道AA的吸收，促进肠道蛋白质的周转。这些发现为支链氨基酸在人类和动物中的使用提供了新的见解。(C)2018 Elsevier Inc.保留所有权利。
Objectives: The aim of this study was to investigate the effects of different branched-chain amino acid (BCAA) ratios on intestinal cell proliferation and protein metabolism.Methods: This in vivo study was conducted in growing pigs (average initial body weight = 9.85 +/- 0.35 kg) exposed to different leucine, isoleucine, and valine (Leu:Ile:Val) ratios (1:1:1, 1:0.75:0.75, 1:0.51:0.63, and 1:0.25:0.25) in 17% crude protein (CP) diets for 45 d to determine intestinal morphology, cell proliferation, and amino acid transporter mRNA expression. This in vitro study was performed in porcine jejunal epithelial cell line to investigate the effects of different BCAA ratios (0, 1:0.25:0.25, 1:0.5:0.5, and 1:1:1) on cell proliferation, AA transporter expression. and protein turnover.Results: Relative to the positive control (Leu:Ile:Val = 1:0.51:0.63, 20% CP), the 1:0.75:0.75 group (17% CP) significantly increased the jejunal villus height, the percentage of proliferating cell nuclear antigen positive cells and decreased the ileal crypt depth (P < 0.05). Moreover, most AA concentrations and AA transporter expression in the jejunal and ileal mucosa in 17% CP diets were increased to the levels similar to those in the positive control. Additionally, this in vitro study showed that the BCAA ratio of 1:0.25:0.25 significantly increased intestinal cell proliferation, mRNA expression of AA transporters, AA concentrations, and protein turnover (P < 0.05).Conclusions: These findings suggest that optimal BCAA ratio (in vivo = 1:0.75:0.75; in vitro = 1:0.25:0.25) would improve intestinal morphology and cell proliferation, increase intestinal AA absorption through mediating expression of intestinal AA transporters, and promote intestinal protein turnover. The findings yield new insights into the use of BCAAs in humans and animals. (C) 2018 Elsevier Inc. All rights reserved.