Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells
Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells
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DOI：
10.4012/dmj.2020-390
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发表时间：
2021-12-01
影响因子：
2.5
通讯作者：
Nikaido, Toru
中科院分区：
文献类型：
--
作者：
Ishigure, Hiroshi;Kawaki, Harumi;Nikaido, Toru
大量研究表明，牙科修复材料中持续释放的离子具有酸缓冲能力，防止牙釉质脱矿，抑制细菌粘附。在此，从表面预反应的玻璃离子（S-PRG）填料释放的离子的释放行为和生物响应性进行了研究，在不同类型的媒体的基础上，人牙髓源性干细胞（hDPSC）的反应。将hDPSC在用不同量的细胞培养基稀释的S-PRG洗脱液中培养1-7天。S-PRG释放出多种离子，如F-、Sr 2+、Na+、Al 3+、BO 33-和SiO 32-。洗脱离子的平衡根据稀释度和溶剂而不同，这进而影响hDPSC的细胞毒性、细胞形态、细胞增殖和烷烃磷酸酶活性等特性。结果表明，定制的S-PRG填料洗脱液可以设计和制备应用于牙科实践。
Numerous studies have shown that the sustained release of ions from dental restorative materials have acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, the release behavior and bioresponsiveness of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types of media based on human dental pulp-derived stem cell (hDPSC) responses. The hDPSCs were cultured for 1-7 days in S-PRG eluates diluted with varying amounts of cell culture media. S-PRG released several types of ions, such as F-, Sr2+, Na+, Al3+, BO33-, and SiO32-. The balance of eluted ions differed depending on the dilution and solvent, which in turn affected the cytotoxicity, cell morphology, cell proliferation, and alkane phosphatase activity of hDPSCs, among other properties. The results suggest that tailored S-PRG filler eluates could be designed and prepared for application in dental practice.