ESI-QqTOF-MS/MS and APCI-IT-MS/MS analysis of steroid saponins from the rhizomes of Dioscorea panthaica
ESI-QqTOF-MS/MS and APCI-IT-MS/MS analysis of steroid saponins from the rhizomes of Dioscorea panthaica
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DOI：
10.1002/jms.988
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发表时间：
2006-01-01
影响因子：
2.3
通讯作者：
Ding, LS
中科院分区：
文献类型：
--
作者：
Li, R;Zhou, Y;Ding, LS
采用高分辨四极杆飞行时间质谱沿着电喷雾电离源（ESI-QqTOF-MS）技术，对13种从黄藤根茎中提取的甾体皂苷类化合物进行了准确的分子量测定，并得到了相应的分子式。为了阐明D.采用电喷雾电离-QqTOF-MS/MS技术对10个道地药材进行了结构分析，并采用大气压化学电离-离子阱串联质谱（APCI-IT-MS/MS）技术对13个甾体皂苷类化合物进行了结构分析。panthaica。通过对串联质谱数据的分析，确定了螺甾烷醇和呋甾烷醇甾体皂苷的诊断碎片离子。panthaica检测为m/z 271.2056和253.1951。此外，还检测到了4对异构体，并对其中4种未知甾体皂苷的可能结构进行了推测。潘塞卡猜测道。ESI-TOF和APCI-MSn已被证明是研究甾体皂苷裂解机理和快速测定提取混合物中天然甾体皂苷的有效工具，从而避免了繁琐的衍生和分离步骤。版权所有（c）2006约翰威利父子有限公司。
Using high-resolution quadrupole time-of-flight mass spectrometry along with an electrospray ionization source (ESI-QqTOF-MS), accurate molecular weights of 13 steroid saponins extracted from the rhizomes of Dioscorea panthaica were acquired and the corresponding molecular formulae obtained. In order to elucidate the fragmentation pathways of steroid saponins in D. panthaica, 10 authentic samples were investigated using ESI-QqTOF-MS/MS. In addition, atmospheric pressure chemical ionization mass spectrometry combined with ion trap tandem mass spectrometry (APCI-IT-MS/MS) was used to analyze the structures of 13 steroid saponins in D. panthaica. Through the analysis of their tandem mass data, diagnostic fragment ions of the spirostanol and furostanol steroid saponins in D. panthaica were detected as m/z 271.2056 and 253.1951. In addition, four pairs of isomers were detected and the possible structures of four unknown steroid saponins in D. panthaica speculated. ESI-TOF and APCI-MSn have proved to be effective tools for research on fragmentation mechanism of steroid saponins and the rapid determination of native steroid saponins in extract mixture, thereby avoiding tedious derivation and separation steps. Copyright (c) 2006 John Wiley & Sons, Ltd.