Amygdalohippocampal Area Neurons That Project to the Preoptic Area Mediate Infant-Directed Attack in Male Mice
Amygdalohippocampal Area Neurons That Project to the Preoptic Area Mediate Infant-Directed Attack in Male Mice
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DOI：
10.1523/jneurosci.0438-19.2020
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发表时间：
2020-04
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通讯作者：
Keiichiro Sato;Yumi Hamasaki;K. Fukui;Kazuki Ito;Kazunari Miyamichi;M. Minami;Taiju Amano
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文献类型：
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作者：
Keiichiro Sato;Yumi Hamasaki;K. Fukui;Kazuki Ito;Kazunari Miyamichi;M. Minami;Taiju Amano
雄性动物可能会对婴儿表现出不同的行为：攻击或养育。这些行为是在内部状态的影响下由幼犬刺激触发的，包括荷尔蒙环境和/或社会经验。收敛的数据表明，内侧视前区（MPOA）有助于对幼犬的行为选择。然而，整合刺激如何影响MPOA依赖的行为选择的神经机制仍不清楚。雄性动物可能会对婴儿表现出不同的行为：攻击或养育。这些行为是在内部状态的影响下由幼犬刺激触发的，包括荷尔蒙环境和/或社会经验。收敛的数据表明，内侧视前区（MPOA）有助于对幼犬的行为选择。然而，整合刺激如何影响MPOA依赖的行为选择的神经机制仍不清楚。在这里，我们专注于杏仁核海马区（AHi）项目MPOA和催产素受体，激素受体介导的社会行为对幼崽。我们描述了激活MPOA投射AHi神经元在雄性小鼠的社会接触与幼崽。使用TRIO方法的输入映射显示，MPOA投射AHi神经元从几个区域接收突出的输入，包括丘脑、下丘脑和嗅觉皮层。电生理和组织学分析表明，催产素调节MPOA投射AHi神经元的抑制性突触反应。此外，AHi形成MPOA的兴奋性单突触，并且MPOA投射AHi神经元的药理学激活仅增强攻击行为，但不增强亲代行为。有趣的是，这种促进行为与雄性小鼠的社会经验有关。总的来说，我们的研究结果确定了MPOA的突触前伙伴，可以整合感觉输入和激素状态，并触发小狗定向攻击。内侧视前区（MPOA）在父母行为中起着关键作用，如运动控制，动机和社会互动。MPOA投射到多个大脑区域，这些投射有助于父母行为中的几个神经控制。相比之下，MPOA的投入如何受到社会和环境信息的调节，人们知之甚少。在这项研究中，我们集中在杏仁核海马区（AHi）连接到MPOA和催产素受体表达。我们证明了由MPOA投射AHi神经元的激活触发的父母行为的表达的中断。这种行为不仅受到催产素的调节，还受到来自多个区域的神经输入的调节。
Male animals may show alternative behaviors toward infants: attack or parenting. These behaviors are triggered by pup stimuli under the influence of the internal state, including the hormonal environment and/or social experiences. Converging data suggest that the medial preoptic area (MPOA) contributes to the behavioral selection toward the pup. However, the neural mechanisms underlying how integrated stimuli affect the MPOA-dependent behavioral selection remain unclear. Male animals may show alternative behaviors toward infants: attack or parenting. These behaviors are triggered by pup stimuli under the influence of the internal state, including the hormonal environment and/or social experiences. Converging data suggest that the medial preoptic area (MPOA) contributes to the behavioral selection toward the pup. However, the neural mechanisms underlying how integrated stimuli affect the MPOA-dependent behavioral selection remain unclear. Here we focus on the amygdalohippocampal area (AHi) that projects to MPOA and expresses oxytocin receptor, a hormone receptor mediating social behavior toward pups. We describe the activation of MPOA-projection AHi neurons in male mice by social contact with pups. Input mapping using the TRIO method reveals that MPOA-projection AHi neurons receive prominent inputs from several regions, including the thalamus, hypothalamus, and olfactory cortex. Electrophysiological and histologic analysis demonstrates that oxytocin modulates inhibitory synaptic responses on MPOA-projection AHi neurons. In addition, AHi forms the excitatory monosynapse to MPOA, and pharmacological activation of MPOA-projection AHi neurons enhances only aggressive behavior, but not parental behavior. Interestingly, this promoted behavior was related to social experience in male mice. Collectively, our results identified a presynaptic partner of MPOA that can integrate sensory input and hormonal state, and trigger pup-directed aggression. SIGNIFICANCE STATEMENT The medial preoptic area (MPOA) plays critical roles in parental behavior, such as motor control, motivation, and social interaction. The MPOA projects to multiple brain regions, and these projections contribute to several neural controls in parental behavior. In contrast, how inputs to MPOA are regulated by social and environmental information is poorly understood. In this study, we focus on the amygdalohippocampal area (AHi) that connects to MPOA and expresses oxytocin receptor. We demonstrate the disruption of the expression of parental behavior triggered by the activation of MPOA-projection AHi neurons. This behavior may be regulated not only by oxytocin but also by neural input from several regions.