Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.
Use of tandem affinity chromatography for purification of cannabinoid receptor CB₂.
复制标题
使用串联亲和层析纯化大麻素受体 CBα。
DOI：
10.1007/978-1-4939-1034-2_9
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yeliseev,AlexeiA
中科院分区：
文献类型：
--
作者：
Locatelli-Hoops,SilviaC;Yeliseev,AlexeiA
Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion that lack either N- or C-terminal tags. Here, we describe the use of two small affinity tags, a poly-histidine tag and a Strep-tag for expression and purification of the human cannabinoid receptor CB2, an integral membrane G protein-coupled receptor.
