A novel glycoprotein from Streptomyces sp. triggers early responses of plant defense
A novel glycoprotein from Streptomyces sp. triggers early responses of plant defense
复制标题
DOI：
10.1016/j.pestbp.2020.104719
复制
发表时间：
2021-01-01
影响因子：
4.7
通讯作者：
Feng, Juntao
中科院分区：
文献类型：
--
作者：
Han, Lirong;Sun, Yubo;Feng, Juntao
链霉菌一种新的糖蛋白GP-1。ZX01具有植物免疫诱导作用。GP-1处理的植株表现出增强的系统抗性，显著减少了烟草叶片上的TMV病斑，但其抗病毒机制尚不清楚。本研究以烟草为材料，研究了GP-1诱导烟草对病毒的早期防御反应，包括Ca~(2+)内流、愈伤组织堆积、氧化猝发、过敏性反应、细胞程序性死亡、一氧化氮(NO)升高和气孔关闭等，并对GP-1诱导烟草产生病毒抗性的机制进行了初步探讨。结果表明，GP-1能迅速诱导烟草叶片和悬浮细胞内[Ca~(2+)](Cyt)的产生，随后引起活性氧(ROS)和NO的升高。转录组分析表明，经GP-1处理的本氏沼虾与对照相比，其基因表达水平存在明显差异，并且表现出显着上调和丰富的防御和免疫反应等途径。与典型的病原菌相关分子模式相似，GP-1诱导了胼胝质沉积和气孔关闭，形成了抵御病原菌入侵的防御屏障。防御相关基因的表达进一步证实了上述结论。通过转录组分析和水杨酸(SA)和茉莉酸(JA)含量的分析，GP-1通过提高SA和JA含量，增强植物次生代谢产物活性，通过产生抗病毒活性促进病程相关蛋白的系统积累，增强了烟草对病毒的抗性。
GP-1, a novel glycoprotein from Streptomyces sp. ZX01 has a plant immunity-inducing effect. GP-1-treated plants exhibited enhanced systemic resistance with a significant reduction in TMV lesions on tobacco leaves, but its antiviral mechanism remains unclear. In this study, early plant defense-related responses, such as Ca2+ influx, callose apposition, oxidative burst, hypersensitive response, programmed cell death, increase in nitric oxide (NO), and stomatal closure, were investigated under GP-1 treatment, and the mechanism of how GP-1 induces viral resistance in Nicotiana benthamiana was studied. Results showed that GP-1 induced [Ca2+](cyt) rapidly in tobacco leaves and suspended cells, followed by reactive oxygen species (ROS) and NO elevation. Transcriptome analysis showed significant differences in expression levels between the GP-1-treated N. benthamiana and the control and showed significantly upregulated and enriched pathways including defense and immune reaction. Similar to typical pathogen-associated molecular patterns, GP-1 induced callose deposition and stomatal closure to form defense barriers against pathogen invasion. The expression of defense-related genes further confirmed the above conclusions. By analyzing transcriptome in N. benthamiana and the contents of salicylic acid (SA) and jasmonic acid (JA), GP-1 enhanced viral resistance of tobacco by improving the SA and JA contents, strengthening plant secondary metabolites activities, promoting systemic accumulation of pathogenesis-related proteins in TMV- inoculated tobacco there by producing antiviral activity.