Synthesis of a selenocysteine-containing peptide by native chemical ligation
Synthesis of a selenocysteine-containing peptide by native chemical ligation
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DOI：
10.1021/ol015712o
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发表时间：
2001-05-03
期刊：
影响因子：
5.2
通讯作者：
van der Donk, WA
中科院分区：
文献类型：
--
作者：
Gieselman, MD;Xie, LL;van der Donk, WA
[图解]描述了一种合成硒半胱氨酸衍生物和含硒半胱氨酸肽的新方法。制备了Fmoc-Sep甲氧基苄基硒半胱氨酸(1)，并用于N端无保护的硒半胱氨酸的固相合成肽，随后与多肽硫酯的天然化学连接得到了对应于核糖核苷酸还原酶C端的17聚体，用硒半胱氨酸代替半胱氨酸。
[GRAPHICS]A new method for the synthesis of selenocysteine derivatives and selenocysteine-containing peptides is described. Fmoc-Sep methoxybenzylselenocysteine (1) was prepared and used for solid-phase synthesis of peptides with an N-terminal unprotected selenocysteine, Subsequent native chemical ligation with a peptide thioester provided a 17-mer that corresponds to the C-terminus of ribonucleotide reductase with selenocysteine in place of cysteine.