PSTha5a23, a candidate effector from the obligate biotrophic pathogen Puccinia striiformis f. sp tritici, is involved in plant defense suppression and rust pathogenicity
PSTha5a23, a candidate effector from the obligate biotrophic pathogen Puccinia striiformis f. sp tritici, is involved in plant defense suppression and rust pathogenicity
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PSTha5a23，来自专性生物营养病原体条锈菌 f 的候选效应子。
DOI：
10.1111/1462-2920.13610
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发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
--
作者：
Cheng, Yulin;Wu, Kuan;Kang, Zhensheng
在寄主植物侵染过程中，病原菌可以传递与毒力相关的效应蛋白来促进植物的敏感性。然而，对专性生物营养病原菌Puccinia striiformis f.sp.的效应功能知之甚少。小麦赤霉菌(Tritici，PST)是小麦生产上的重要病原菌。在这里，他们报告了他们在植物内高度诱导的PST候选效应器PSTha5a23上的发现。PSTha5a23基因是PST所特有的，具有较低的种内多态性。它有一个功能性的N末端信号肽，感染后被转运到宿主细胞质中。在烟草中过表达PSTha5a23可以抑制Bax、PAMP-INF1和两个抗性相关的丝裂原激活蛋白激酶(MKK1和NPK1)引发的细胞程序性死亡。PSTha5a23在小麦中的过表达也抑制了模式触发免疫(PTI)相关的愈伤组织沉积。此外，PSTha5a23的沉默没有改变PST的毒力表型，但PSTha5a23的过表达显著提高了小麦的PST毒力。这些结果表明，PST候选效应子PSTha5a23在植物防御抑制和锈病致病中起着重要作用，也突显了植物基因过表达作为研究专性生物营养型病原菌效应物的作用。
During the infection of host plants, pathogens can deliver virulence-associated 'effector' proteins to promote plant susceptibility. However, little is known about effector function in the obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) that is an important fungal pathogen in wheat production worldwide. Here, they report their findings on an in planta highly induced candidate effector from Pst, PSTha5a23. The PSTha5a23 gene is unique to Pst and shows a low level of intra-species polymorphism. It has a functional N-terminal signal peptide and is translocated to the host cytoplasm after infection. Overexpression of PSTha5a23 in Nicotiana benthamiana was found to suppress the programmed cell death triggered by BAX, PAMP-INF1 and two resistance-related mitogen-activated protein kinases (MKK1 and NPK1). Overexpression of PSTha5a23 in wheat also suppressed pattern-triggered immunity (PTI)-associated callose deposition. In addition, silencing of PSTha5a23 did not change Pst virulence phenotypes; however, overexpression of PSTha5a23 significantly enhanced Pst virulence in wheat. These results indicate that the Pst candidate effector PSTha5a23 plays an important role in plant defense suppression and rust pathogenicity, and also highlight the utility of gene overexpression in plants as a tool for studying effectors from obligate biotrophic pathogens.