Aromatic DNA adducts in human bone marrow and peripheral blood leukocytes.
Aromatic DNA adducts in human bone marrow and peripheral blood leukocytes.
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人骨髓和外周血白细胞中的芳香族 DNA 加合物。
DOI：
10.1093/carcin/7.12.2071
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发表时间：
1986
期刊：
影响因子：
4.7
通讯作者：
P. Grover
中科院分区：
文献类型：
--
作者：
D. Phillips;A. Hewer;P. Grover
正常人骨髓单核细胞和非单核细胞的DNA进行了分析，通过32 P-后标记的芳香族加合物的存在。10个个体中有10个显示存在加合物，其水平为每10（8）个核苷酸1-9个加合物，而在4个人胎儿骨髓样本中未检出。个体间的变化，这些假定的芳香族加合物的模式进行了观察。吸烟者和非吸烟者外周血白色细胞的DNA中也存在类似的加合物，尽管其水平低于骨髓。数据表明，加合物是由于环境暴露于尚未鉴定的遗传毒性剂或制剂所致。
DNA from normal human bone marrow mononuclear and non-mononuclear cells was analysed by 32P-post-labelling for the presence of aromatic adducts. Ten out of 10 individuals showed the presence of adducts at levels of 1-9 adducts per 10(8) nucleotides that were not detected in four samples of human foetal bone marrow. Inter-individual variations in the patterns of these presumed aromatic adducts were observed. Similar adducts were also present in the DNA of peripheral white blood cells of both smokers and non-smokers, although at lower levels than in bone marrow. The data suggest that the adducts result from environmental exposure to an as-yet-unidentified genotoxic agent or agents.