Role of C-elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry
Role of C-elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry
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DOI：
10.1126/science.1155847
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发表时间：
2008-04-25
期刊：
影响因子：
56.9
通讯作者：
Xue, Ding
中科院分区：
文献类型：
--
作者：
Darland-Ransom, Monica;Wang, Xiaochen;Xue, Ding
磷脂在质膜上的不对称分布对于维持细胞完整性和生理学以及调节细胞内信号传导和重要细胞事件（例如凋亡细胞的清除）是至关重要的。磷脂不对称性是如何建立和维持的还不完全清楚。我们报道了秀丽隐杆线虫P型腺苷三磷酸酶同系物达特-1对维持细胞表面磷脂酰丝氨酸（PS）的不对称性至关重要。在缺乏达特-1的动物中，PS异常地暴露在细胞表面上，并且正常的活细胞通过依赖于PSR- 1（一种PS识别吞噬细胞受体）和CED- 1（其有助于识别和吞噬凋亡细胞）的机制随机丢失。因此，达特- 1的功能似乎是防止PS出现在质膜的外叶，PS在细胞表面的异位暴露可能会导致相邻的吞噬细胞去除活细胞。
The asymmetrical distribution of phospholipids on the plasma membrane is critical for maintaining cell integrity and physiology and for regulating intracellular signaling and important cellular events such as clearance of apoptotic cells. How phospholipid asymmetry is established and maintained is not fully understood. We report that the Caenorhabditis elegans P- type adenosine triphosphatase homolog, TAT-1, is critical for maintaining cell surface asymmetry of phosphatidylserine ( PS). In animals deficient in tat-1, PS is abnormally exposed on the cell surface, and normally living cells are randomly lost through a mechanism dependent on PSR- 1, a PS- recognizing phagocyte receptor, and CED- 1, which contributes to recognition and engulfment of apoptotic cells. Thus, tat- 1 appears to function in preventing appearance of PS in the outer leaflet of plasma membrane, and ectopic exposure of PS on the cell surface may result in removal of living cells by neighboring phagocytes.