PATTERNS OF ACTH AND CORTISOL PULSATILITY OVER 24 HOURS IN NORMAL MALES AND FEMALES
PATTERNS OF ACTH AND CORTISOL PULSATILITY OVER 24 HOURS IN NORMAL MALES AND FEMALES
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DOI：
10.1111/j.1365-2265.1990.tb03758.x
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发表时间：
1990-01-01
影响因子：
3.2
通讯作者：
LONDON, DR
中科院分区：
文献类型：
--
作者：
HORROCKS, PM;JONES, AF;LONDON, DR
研究人员通过留置套管在 24 小时内每 15 分钟采样一次，研究了 10 名健康受试者（卵泡早期 5 名男性、5 名女性，总体年龄范围 21-32 岁）的血浆 ACTH 和皮质醇浓度模式。受试者住院、能行走并正常进餐。通过双位点免疫放射测定法测量血浆 ACTH，检测限为 3.9 ng/l (0.9 pmol/l)。豆类的鉴定采用 Clayton 等人的方法。 (1987) 使用严格的标准来最大限度地减少假阳性峰。所有受试者均显示 ACTH 昼夜节律，除一名受试者外，所有受试者的末期发生在 0615 至 0920 小时之间，中值在 9.2 至 18.6 ng/l（2.0 至 4.1 pmol/l）之间。与其他三个 6 小时时段相比，1800 至 2400 小时之间的脉冲明显较少。男性和女性的 ACTH 模式在几个方面存在差异：男性的脉冲数更多（18 vs 10）、平均峰值幅度更大（16.8 ng/l vs 10.3 ng/l）、24 小时曲线下面积更大（350.9 vs 206.6 ng/l h）以及更高的平均水平（14.7 vs 8.6 ng/l）。相比之下，皮质醇模式没有显示出统计学上不同的性别差异。性别差异表明女性肾上腺皮质对 ACTH 的敏感性或可用性更高，或者皮质醇反馈的设定点不同。
Patterns of plasma ACTH and cortisol concentrations were studied in 10 healthy subjects (five male, five female in the early follicular phase, overall age range 21-32 years) by sampling through an indwelling cannula every 15 min for 24 h. The subjects were in hospital, ambulant, and taking normal meals. Plasma ACTH was measured by a two-site immunoradiometric assay with a detection limit of 3.9 ng/l (0.9 pmol/l). Pulses were identified by the method of Clayton et al. (1987) using stringent criteria to minimize false positive peaks. All subjects showed a circadian rhythm of ACTH, the acrophase occurring between 0615 and 0920 h in all but one subject and the mesor value was between 9.2 and 18.6 ng/l (2.0 and 4.1 pmol/l). There were significantly fewer pulses between 1800 and 2400 h compared with the other three 6-h periods. The pattern of ACTH differed between males and females in several respects: more pulses (18 vs 10), greater mean peak amaplitude (16.8 vs 10.3 ng/l), greater area under the 24-h profile (350.9 vs 206.6 ng/l h) and higher mean level (14.7 vs 8.6 ng/l) in the males. In contrast, the cortisol pattern did not show statistically different sex differences. The sex differences suggest greater sensitivity to, or availability of, ACTH to the female adrenal cortex, or different set points in cortisol feedback.