STUDIES OF THE ISOLATION AND VIABILITY OF HUMAN ISLETS OF LANGERHANS
STUDIES OF THE ISOLATION AND VIABILITY OF HUMAN ISLETS OF LANGERHANS
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DOI：
10.1097/00007890-198805000-00024
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发表时间：
1988-05-01
期刊：
影响因子：
6.2
通讯作者：
EGEBJERG, J
中科院分区：
文献类型：
--
作者：
WARNOCK, GL;ELLIS, D;EGEBJERG, J
将34例成人尸体器官供体胰腺分为近端和远端两段，并对各段胰管进行插管。7个腺体近端导管注射胶原酶，7个腺体远端导管注射胶原酶；对段导管灌注胶原酶。然后胰腺通过戏弄、折磨和通过过滤器来分散。近段和远段灌注释放1461。287和2728 .+-。797胰岛/g (.+-. sem) vs . 710。149 （P < 0.05）和1950。注射后636。另外20只胰腺灌注胶原酶，迅速加热至39度。C （n=4）或慢慢加热到37度。C （n=6）或39度。C (n=10)：产率为1625 +-。632, 1320 .+-。116，和2009。277个岛/g。后者的总收益率为76倍。大的；大的；大的M)和85。103个小（6小时）和组织从多个中心获得。新鲜分离的胰岛产生的胰岛素较少
Pancreas obtained from 34 adult human cadaver organ donors was divided into proximal and distal segments, and the duct to each segment was cannulated. Collagenase was injected into the proximal duct of 7 glands and into the distal duct of 7 others; the duct of the opposite segment was perfused with collagenase. The pancreas was then dispersed by teasing, trituration, and passage through filters. Perfused proximal and distal segments released 1461 .+-. 287 and 2728 .+-. 797 islets/g (.+-.SEM) versus 710 .+-. 149 (P < 0.05) and 1950 .+-. 636 after injection. Twenty other pancreases were perfused with collagenase warmed rapidly to 39.degree. C (n=4) or warmed slowly to 37.degree. C (n=6) or 39.degree. C (n=10): the yield was 1625 .+-. 632, 1320 .+-. 116, and 2009 .+-. 277 islets/g respectively. Total yields from the latter were 76 .times. 103 large (>100 .mu.m) and 85 .+-. 103 small (6 hr and tissue obtained from multiple centers. Less insulin was produced by freshly isolated islets, islets