Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process
Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process
复制标题
山核桃（Carya cathayensis）嫁接联合的比较蛋白质组学分析为嫁接过程的响应机制提供了见解
DOI：
10.3389/fpls.2017.00676
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发表时间：
2017-04-27
影响因子：
5.6
通讯作者：
Zheng, Bingsong
中科院分区：
文献类型：
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作者：
Xu, Dongbin;Yuan, Huwei;Zheng, Bingsong
山核桃（Carya cathayensis）是我国广泛栽培的一种具有较高营养价值和经济价值的树种。嫁接大大缩短了幼龄期长度，使山核桃的规模化栽培成为可能。为了揭示这一物种嫁接的反应机制，我们采用了蛋白质组学为基础的方法，以确定差异表达的蛋白质在嫁接过程中的嫁接工会。我们的研究鉴定了3723种蛋白质，其中2518种被定量。共定量了710个差异表达蛋白（DEPs），这些差异表达蛋白涉及各种分子功能和生物学过程。在这些DEPs中，341个在嫁接后7天与对照相比表达上调，369个表达下调。4个生长素相关蛋白表达下调，与其编码基因的转录水平一致。京都基因和基因组百科全书（KEGG）分析表明，“黄酮合成”途径和“淀粉和蔗糖代谢”都显着上调。有趣的是，五个类黄酮生物合成相关的蛋白质，黄烷酮3-羟化酶，肉桂酸4-羟化酶，二氢黄酮醇-4-还原酶，查尔酮合成酶，和查尔酮异构酶，显着上调。进一步的实验证实了接穗中总黄酮含量的显著增加，这表明嫁接联合的形成可能激活了黄酮的生物合成，从而增加了一系列下游次生代谢产物的含量。这一全面的分析提供了基本信息的候选蛋白质和次生代谢途径参与嫁接过程的山核桃。
Hickory (Carya cathayensis), a tree with high nutritional and economic value, is widely cultivated in China. Grafting greatly reduces the juvenile phase length and makes the large scale cultivation of hickory possible. To reveal the response mechanisms of this species to grafting, we employed a proteomics-based approach to identify differentially expressed proteins in the graft unions during the grafting process. Our study identified 3723 proteins, of which 2518 were quantified. A total of 710 differentially expressed proteins (DEPs) were quantified and these were involved in various molecular functional and biological processes. Among these DEPs, 341 were up-regulated and 369 were down-regulated at 7 days after grafting compared with the control. Four auxin-related proteins were down-regulated, which was in agreement with the transcription levels of their encoding genes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the ‘Flavonoid biosynthesis’ pathway and ‘starch and sucrose metabolism’ were both significantly up-regulated. Interestingly, five flavonoid biosynthesis-related proteins, a flavanone 3-hyfroxylase, a cinnamate 4-hydroxylase, a dihydroflavonol-4-reductase, a chalcone synthase, and a chalcone isomerase, were significantly up-regulated. Further experiments verified a significant increase in the total flavonoid contents in scions, which suggests that graft union formation may activate flavonoid biosynthesis to increase the content of a series of downstream secondary metabolites. This comprehensive analysis provides fundamental information on the candidate proteins and secondary metabolism pathways involved in the grafting process for hickory.