GLUTATHIONE SYNTHESIS IN RESPONSE TO CAPTAN - A POSSIBLE MECHANISM FOR RESISTANCE OF BOTRYTIS-CINEREA TO THE FUNGICIDE
GLUTATHIONE SYNTHESIS IN RESPONSE TO CAPTAN - A POSSIBLE MECHANISM FOR RESISTANCE OF BOTRYTIS-CINEREA TO THE FUNGICIDE
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DOI：
10.1016/0048-3575(84)90110-x
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发表时间：
1984-01-01
影响因子：
4.7
通讯作者：
EDGINGTON, LV
中科院分区：
文献类型：
--
作者：
BARAK, E;EDGINGTON, LV
两个B。在施用克菌丹之前，将田间克菌丹抗性和正常类型的灰霉病菌分离物在液体培养基中生长4天。在其生长的第5天的不同时间，分析了其菌丝体和培养基中的谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）。只有轻微的差异，谷胱甘肽水平之间的分离物进行了观察，但菌丝体谷胱甘肽显着差异被发现在响应克菌丹。克菌丹的应用程序后，更少的GSH产生，并在一个较长的滞后期后，由正常类型的真菌相比，耐药菌株。抗性分离物中GSH水平的这种增加可以使更多的非重要硫醇化合物可用于解毒克菌丹，因此，防止杀真菌剂可能对真菌细胞的重要蛋白硫醇造成的损害。
Two B. cinerea isolates, field captan-resistant and normal type, were grown in liquid medium for 4 days prior to captan application. The glutathione (GSH) and oxidized glutathione (GSSG) of their mycelium and medium were analyzed at various times during the 5th day of their growth. Only slight differences in GSH levels between the isolates were observed, but marked differences in mycelial GSH were found in response to captan. Following the application of captan, less GSH was produced and after a longer lag period by the normal type of the fungus compared to the resistant isolate. This increase in the GSH level in the resistant isolate could make more nonvital thiol compound available for detoxifying captan and, therefore, prevent damage the fungicide could cause to the vital protein thiols of fungal cells.