Acute effect of calcium and insulin on hyperfiltration of early diabetes.
Acute effect of calcium and insulin on hyperfiltration of early diabetes.
复制标题
钙和胰岛素对早期糖尿病超滤的急性影响。
DOI：
10.1152/ajpendo.1987.252.1.e13
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Aynedjian,HS
中科院分区：
文献类型：
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作者：
Bank,N;Lahorra,MA;Aynedjian,HS
我们观察了钙对链脲佐菌素治疗的糖尿病大鼠肾脏高滤过的影响。大鼠于注射链脲佐菌素后7~10天取材。肾内注射CaSO4对糖尿病大鼠肾脏的高滤过无影响。在不影响高血糖的剂量下，胰岛素的输注对高滤过也没有影响。然而，当胰岛素和钙一起注入时，肾小球滤过率、单肾单位滤过率、肾小球水压和肾血浆流量迅速下降。对侧对照肾未受影响。维拉帕米对未治疗的糖尿病大鼠无明显作用，但可立即逆转胰岛素加钙引起的血管收缩。类似的肾内胰岛素和钙输注对正常血容量或慢性盐负荷的非糖尿病大鼠没有影响。观察表明，在早期的胰岛素依赖型糖尿病中，肾血管细胞(可能是肾小球前细胞)对钙有高反应性，这种反应需要胰岛素。我们认为，早期胰岛素依赖型糖尿病患者肾血管张力降低可能部分是由于跨血管平滑肌细胞的跨膜钙流动障碍所致。胰岛素似乎是钙离子进入或动员所必需的，以启动糖尿病患者的肾血管平滑肌收缩。
We examined the effect of calcium administration on renal hyperfiltration in streptozotocin-treated diabetic rats. Rats were studied 7–10 days after streptozotocin injection. Intrarenal infusion of CaSO4 in Ringer's solution had no effect on the hyperfiltration of the diabetic kidney. Infusion of insulin in a dose that did not effect hyperglycemia also had no effect on the hyperfiltration. However, when insulin and calcium were infused together, a rapid decrease in glomerular filtration rate, single-nephron filtration rate, glomerular hydraulic pressure, and renal plasma flow occurred. The contralateral control kidney was unaffected. Verapamil infusion had no significant effect in untreated diabetic rats, but immediately reversed the vasoconstriction induced by insulin plus calcium. Similar intrarenal insulin and calcium infusions had no effect in euvolemic or chronically salt-loaded nondiabetic rats. The observations indicate that renal vascular cells (probably preglomerular) are hyperresponsive to calcium in early insulin-dependent diabetes mellitus and that this response requires insulin. We suggest that decreased renal vascular tone in early insulin-dependent diabetes mellitus may be due in part to defective transmembrane calcium flux across vascular smooth muscle cells. Insulin appears to be required for calcium entry or mobilization, to initiate renal vascular smooth muscle contraction in diabetes.