One novel strawberry MADS-box transcription factor FaIVIADS1α acts as a negative regulator in fruit ripening
One novel strawberry MADS-box transcription factor FaIVIADS1α acts as a negative regulator in fruit ripening
复制标题
DOI：
10.1016/j.scienta.2017.09.042
复制
发表时间：
2018-01-03
影响因子：
4.3
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Lu, Wenjing;Chen, Jingxin;Luo, Zisheng
MADS-box 基因编码高度保守的 DNA 结合转录因子，可以调节植物的发育过程。迄今为止，尚未对非更年期水果中的 FaMADS1 α 进行功能分析。在本研究中，克隆了一种草莓（Fragaria x ananassa）MADS-box 基因 FaMADS1 α，并分析了其组织特异性表达谱。 FaMADS1α在花瓣和果实中表达，其表达量在果实成熟过程中显着下降。多重序列比对表明 FaMADS1 α 蛋白表现出保守的 MADS_MEF2_like 基序、K-box 区域、MADS 结构域、SRF-TF 基序和 ARG80 结构域。系统发育分析表明 FaMADS1 alpha 属于 SEP1/2 Glade。植物激素脱落酸（ABA）抑制FaMADS1α表达，但加速成熟过程和花青素相关基因表达，而吲哚-3-乙酸（MA）诱导FaMADS1α表达，具有延迟成熟表型并减少花青素相关基因表达。与对照相比，pBI121 载体介导的 FaMADS1 α a 过度表达降低了四种花青素相关基因的表达，并延迟了果实成熟。结果表明，FaMADS1 α 通过抑制 FaPAL6、FaCHS、FaDFR 和 FaANS 对草莓果实花青素的积累发挥负面作用。
MADS-box genes, which encode highly conserved DNA-binding transcriptional factors, can regulate developmental processes in plants. FaMADS1 alpha has not been functionally analyzed in non-climacteric fruit to date. In this study, one strawberry (Fragaria x ananassa) MADS-box gene, FaMADS1 alpha, was cloned and its tissue-specific expression profiles were analyzed. FaMADS1 alpha expressed in petal and fruit, and its expression levels decreased significantly during fruit ripening. Multiple sequence alignments suggested that FaMADS1 alpha protein exhibited conserved MADS_MEF2_like motif, K-box region, MADS domain, SRF-TF motif and ARG80 domain. Phylogenetic analysis revealed that FaMADS1 alpha belonged to the SEP1/2 Glade. Phytohormone abscisic acid (ABA) suppressed FaMADS1 alpha expression but accelerated the ripening process and anthocyanin-related gene expressions, while indole-3-acetic acid (MA) induced FaMADS1 alpha expression with delayed ripening phenotype and reduced expressions of anthocyanin-related genes. The pBI121 vector-mediated over-expression of FaMADS1 alpha a reduced the expressions of four anthocyanin-related genes and delayed fruit ripening compared to the control. Results suggested that FaMADS1 alpha played a negative role in anthocyanin accumulation of strawberry fruit via repressing FaPAL6, FaCHS, FaDFR and FaANS.