Changes in lipid values and lipoprotein patterns of serum samples contaminated with bacteria.
Changes in lipid values and lipoprotein patterns of serum samples contaminated with bacteria.
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被细菌污染的血清样本的脂质值和脂蛋白模式的变化。
DOI：
10.1093/ajcp/59.6.829
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发表时间：
1973
影响因子：
3.5
通讯作者：
H. Friedman
中科院分区：
文献类型：
--
作者：
H. Schwertner;H. Friedman
在邮寄的用于脂质分析的血清样本中，发现3%的污染物为产磷脂酶c杆菌。污染导致血清甘油三酯值和乳糜微粒明显增加，血清磷脂值和α、β和β前脂蛋白相应降低。用产气荚膜梭菌分离的磷脂酶C证实了污染血清的变化。结果表明，磷脂分子的磷部分暴露在水环境中，对脂蛋白的稳定特性和电泳特性至关重要。乙二胺四乙酸可抑制磷脂酶C，对保持血清脂质和脂蛋白的结构完整性有效。
Phospholipase C-producing bacilli were found as contaminants in 3% of serum samples received by mail for lipid analysis. The contamination results in apparent increases in serum triglyceride values and chylomicrons, with corresponding decreases in serum phospholipid values and alpha, beta, and prebeta lipoproteins. The changes in contaminated sera were confirmed with phospholipase C isolated from Clostridium perfringens . The results suggest that the phosphorus portion of the phospholipid molecule is exposed to the aqueous environment and is essential for the stabilization characteristics and electrophoretic properties of lipoproteins. Ethylenediamine tetraacetic acid, which inhibits phospholipase C, was found to be effective in preserving the structural integrity of serum lipids and lipoproteins.