L-glutamine therapy reduces endothelial adhesion of sickle red blood cells to human umbilical vein endothelial cells.
L-glutamine therapy reduces endothelial adhesion of sickle red blood cells to human umbilical vein endothelial cells.
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DOI：
10.1186/1471-2326-5-4
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发表时间：
2005-07-25
期刊：
影响因子：
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通讯作者：
Tanaka, Kouichi R
中科院分区：
文献类型：
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作者：
Niihara, Yutaka;Matsui, Neil M;Tanaka, Kouichi R
背景技术背景：我们先前已经证明，口服L-谷氨酰胺治疗可改善镰状红细胞（RBC）的烟酰胺腺苷二核苷酸（NAD）氧化还原电位。在进一步分析谷氨酰胺治疗镰状细胞性贫血患者的基础上，研究了谷氨酰胺对镰状红细胞与人脐静脉内皮细胞（HUVEC）粘附的影响。实验的第一部分是用5名成年镰状细胞性贫血患者的血液样本进行的，这些患者一直在服用L-谷氨酰胺治疗至少4周，剂量为30克/天，与患者对照组相比。在实验的第二部分中，对6例镰状细胞性贫血患者进行了纵向研究。其中5名患者每天口服L-谷氨酰胺30克，1名患者未接受治疗作为对照。结果：在第一项研究中，用自体血浆孵育的细胞与内皮细胞的平均粘附力，治疗组为0.97 ± 0.45，未治疗组为1.91 ± 0.53（p < 0.02）。与脂多糖（LPS）孵育的细胞类似，治疗组与内皮细胞的平均粘附力为1.39 +/- 0.33，未治疗组为2.80 +/- 0.47（p < 0.001）。与纵向实验，平均减少粘附内皮细胞为1.13 +/- 0.21（p < 0.001），而对照组患者有轻微增加粘附内皮cells.CONCLUSION：在这些研究中，口服L-谷氨酰胺管理一致导致改善镰状红细胞粘附HUVEC。这些数据表明L-谷氨酰胺在镰状细胞病中具有积极的生理作用。
BACKGROUND: We have previously demonstrated that therapy with orally administered L-glutamine improves nicotinamide adenosine dinucleotide (NAD) redox potential of sickle red blood cells (RBC). On further analysis of L-glutamine therapy for sickle cell anemia patients, the effect of L-glutamine on adhesion of sickle RBC to human umbilical vein endothelial cells (HUVEC) was examined.METHODS: The first part of the experiment was conducted with the blood samples of the 5 adult sickle cell anemia patients who had been on L-glutamine therapy for at least 4 weeks on a dosage of 30 grams per day compared to those of patient control group. In the second part of the experiment 6 patients with sickle cell anemia were studied longitudinally. Five of these patients were treated with oral L-glutamine 30 grams daily and one was observed without treatment as the control. t-test and paired t-test were used for determination of statistical significance in cross-sectional and longitudinal studies respectively.RESULTS: In the first study, the mean adhesion to endothelial cells with the autologous plasma incubated cells were 0.97 +/- 0.45 for the treated group and 1.91 +/- 0.53 for the nontreated group (p < 0.02). Similarly with lipopolysaccharide (LPS) incubated cells the mean adhesion to endothelial cells were 1.39 +/- 0.33 for the treated group and 2.80 +/- 0.47 for the untreated group (p < 0.001). With the longitudinal experiment, mean decrease in the adhesion to endothelial cells was 1.13 +/- 0.21 (p < 0.001) for the 5 treated patients whereas the control patient had slight increase in the adhesion to endothelial cells.CONCLUSION: In these studies, oral L-glutamine administration consistently resulted in improvement of sickle RBC adhesion to HUVEC. These data suggest positive physiological effects of L-glutamine in sickle cell disease.