Quantitative SLM-based Differential Interference Contrast imaging.
Quantitative SLM-based Differential Interference Contrast imaging.
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DOI：
10.1364/oe.18.014063
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发表时间：
2010-06
期刊：
影响因子：
3.8
通讯作者：
T. Mcintyre;C. Maurer;Stephanie Fassl;Saranjam Khan;S. Bernet;M. Ritsch-Marte
中科院分区：
文献类型：
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作者：
T. Mcintyre;C. Maurer;Stephanie Fassl;Saranjam Khan;S. Bernet;M. Ritsch-Marte
我们描述了利用空间光调制器（SLM）作为光路中的灵活傅里叶滤波器的定量微分干涉衬度（DIC）显微术的实现。实验装置允许以视频速率全电子采集多个相移DIC图像，分析这些图像以产生样品的光路长度变化。通过反演聚苯乙烯微球在油中的相位分布，分析了该方法的分辨率，并将其应用于生物样品的定量成像。通过对SLM上显示的衍射结构进行重新编程，可以在同一相机芯片的不同区域中同时记录整组相移DIC图像。这使得定量快照成像的样品，这有应用程序的动态过程的调查。
We describe the implementation of quantitative Differential Interference Contrast (DIC) Microscopy using a spatial light modulator (SLM) as a flexible Fourier filter in the optical path. The experimental arrangement allows for the all-electronic acquisition of multiple phase shifted DIC-images at video rates which are analyzed to yield the optical path length variation of the sample. The resolution of the technique is analyzed by retrieving the phase profiles of polystyrene spheres in immersion oil, and the method is then applied for quantitative imaging of biological samples. By reprogramming the diffractive structure displayed at the SLM it is possible to record the whole set of phase shifted DIC images simultaneously in different areas of the same camera chip. This allows for quantitative snap-shot imaging of a sample, which has applications for the investigation of dynamic processes.